Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies

cope : Evidence suggests that the dietary consumption of plant extracts containing polyphenols might help prevent the onset of cancers of the gastrointestinal tract. In the present study, the chemopreventive and antiproliferative efficacy of a grapevine shoot extract (Vineatrol®30) containing resveratrol and resveratrol oligomers was investigated in vivo and in vitro. Methods and results : The in vivo study was performed using ApcMin mice on a high-fat diet, which represents a model of human adenomatous polyposis, while the potential of the extract as well as some of its isolated constituents to inhibit intestinal adenoma cell proliferation in vitro was investigated using APC10.1 cells derived from an ApcMin mouse. Vineatrol®30 at a low (2.3 mg/kg diet) or high dose (476 mg/kg diet) reduced the adenoma number in male and adenoma volume in female animals. Furthermore, Vineatrol®30 as well as resveratrol and two resveratrol tetramers compromised the expansion of APC10.1 cells by reducing cell number, inducing cell cycle arrest, cellular senescence and apoptosis. However, except for the extract, none of the isolated resveratrol oligomers was more efficacious than resveratrol in these cells. Conclusion : Vineatrol®30 may merit further investigation as a potential dietary gastrointestinal cancer chemopreventive agent in humans. This article is protected by copyright. All rights reserved