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Synergistic Antitumor Activity of Gramicidin/Lipophilic Bismuth Nanoparticles (BisBAL NPs) on Human Cervical Tumor Cells
- Source :
- Frontiers in Nanotechnology, Vol 3 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- The objective of this study was to study the synergistic antitumor effect of lipophilic bismuth nanoparticles (BisBAL NPs) with the antibiotic solution Neo-Poly gramicidin on human cervical tumor cells. The effect of BisBAL NPs and Neo-Poly gramicidin solution on cervical cancer cell line (HeLa) was determined by the MTT cell viability assay and fluorescence microscopy. After a 24-h exposure to 0.1× Neo-Poly gramicidin HeLa cell growth decreased 94%. Fluorescence microscopy confirmed the antitumor effect cell death was higher among treated than among non-treated cells cells. Individually, gramicidin (0.04 mg/mL) inhibited HeLa tumor cell growth most (40%), and neomycin (0.04 mg/mL) least (21%). Gramicidin (0.3 mg/mL) in combination with different concentrations (1–150 μM) of BisBAL NPs had a synergistic antitumor effect against HeLa cells, reaching an < 86% tumor growth inhibition. As far as we know, we are the first to describe the antitumor activity of the antibiotic Neo-Poly gramicidin on a human cervical cancer cell line. The action mechanism of gramicidin/BisBAL NP is based on a strong damage on cell membrane and nucleus of tumor cells. A synergistic effect of gramicidin with BisBAL NPs may be useful as an alternative therapy for cervical cancer patients.
- Subjects :
- Programmed cell death
cervical cancer
02 engineering and technology
lcsh:Chemical technology
Cell membrane
HeLa
gramicidin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Fluorescence microscope
medicine
antitumor activity
lcsh:TP1-1185
Viability assay
biology
Cell growth
bismuth nanoparticles
neomycin
technology, industry, and agriculture
polymyxin B
Neomycin
021001 nanoscience & nanotechnology
biology.organism_classification
Molecular biology
medicine.anatomical_structure
chemistry
030220 oncology & carcinogenesis
Gramicidin
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 26733013
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Frontiers in Nanotechnology
- Accession number :
- edsair.doi.dedup.....104bb2865e442336d7e0dbec3a51b008
- Full Text :
- https://doi.org/10.3389/fnano.2021.633604