Back to Search
Start Over
Liver proteomics of gilthead sea bream (Sparus aurata) exposed to cold stress
- Source :
- Journal of Thermal Biology. 82:234-241
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The gilthead sea bream (Sparus aurata, L.) is very sensitive to low temperatures, which induce fasting and reduced growth performances. There is a strong interest in understanding the impact of cold on fish metabolism to foster the development and optimization of specific aquaculture practices for the winter period. In this study, an 8 week feeding trial was carried out on gilthead sea bream juveniles reared in a Recirculated Aquaculture System (RAS) by applying a temperature ramp in two phases of four weeks each: a cooling phase from 18 °C to 11 °C and a cold maintenance phase at 11 °C. Liver protein profiles were evaluated with a shotgun proteomics workflow based on filter-aided sample preparation (FASP) and liquid chromatography-mass spectrometry (LC-ESI-Q-TOF MS/MS) followed by label-free differential analysis. Along the whole trial, sea breams underwent several changes in liver protein abundance. These occurred mostly during the cooling phase when catabolic processes were mainly observed, including protein and lipid degradation, together with a reduction in protein synthesis and amino acid metabolism. A decrease in protein mediators of oxidative stress protection was also seen. Liver protein profiles changed less during cold maintenance, but pathways such as the methionine cycle and sugar metabolism were significantly affected. These results provide novel insights on the dynamics and extent of the metabolic shift occurring in sea bream liver with decreasing water temperature, supporting future studies on temperature-adapted feed formulations. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD011059.
- Subjects :
- Fish Proteins
Proteomics
0106 biological sciences
Physiology
030310 physiology
Carbohydrate metabolism
010603 evolutionary biology
01 natural sciences
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Methionine
Aquaculture
Tandem Mass Spectrometry
Protein biosynthesis
Animals
Food science
Shotgun proteomics
0303 health sciences
Catabolism
business.industry
Cold-Shock Response
Metabolism
Sea Bream
Liver
chemistry
General Agricultural and Biological Sciences
business
Metabolic Networks and Pathways
Developmental Biology
Subjects
Details
- ISSN :
- 03064565
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Journal of Thermal Biology
- Accession number :
- edsair.doi.dedup.....103189446cbe1ddcbecf097fc3eca300