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Retracing the in vivo haematopoietic tree using single-cell methods
- Source :
- FEBS Letters, FEBS Letters, Wiley, 2016, 590 (22), pp.4068-4083. ⟨10.1002/1873-3468.12299⟩, FEBS Letters, 2016, 590 (22), pp.4068-4083. ⟨10.1002/1873-3468.12299⟩
- Publication Year :
- 2016
-
Abstract
- International audience; The dynamic process by which self-­‐renewing stem cells and their offspring proliferate and differentiate to create the erythroid, myeloid and lymphoid lineages of the blood system has long since been an important topic of study. A range of recent single cell and family-­‐tracing methodologies such as massively parallel single-­‐cell RNA-­‐sequencing, mass cytometry, integration site barcoding, cellular barcoding and transposon barcoding are enabling unprecedented analysis, dissection and re-­‐evaluation of the hematopoietic tree. In addition to the substantial experimental advances, these new techniques have required significant theoretical development in order to make biological deductions from their data. Here we review these approaches from both an experimental and inferential point of view, considering their discoveries to date, their capabilities, limitations and opportunities for further development.
- Subjects :
- 0301 basic medicine
mass cytometry
Cellular differentiation
Biophysics
Computational biology
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Biochemistry
03 medical and health sciences
hematopoietic tree
lineage tracing
Single-cell analysis
Structural Biology
single cells
Genetics
Humans
Mass cytometry
Cell Lineage
Myeloid Cells
Lymphocytes
Molecular Biology
Massively parallel
Erythroid Precursor Cells
Cell Proliferation
RNA sequencing
Cell Differentiation
Cell Biology
Hematopoietic Stem Cells
barcoding
Tree (data structure)
Haematopoiesis
030104 developmental biology
Stem cell
Single-Cell Analysis
Subjects
Details
- ISSN :
- 18733468 and 00145793
- Volume :
- 590
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....101a97a9fea939c36b2007e00a827f40