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Nasal Colonization of Humans with Occupational Exposure to Raw Meat and to Raw Meat Products with Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus
- Source :
- Toxins, Toxins, Vol 11, Iss 4, p 190 (2019), Volume 11, Issue 4
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely disseminated as a nasal colonizer of conventionally raised livestock and of humans subjected to occupational exposure. Reports on contamination of raw meat raise the question as to whether occupationally exposed food handlers are at particular risk of nasal colonization by LA-MRSA. Here, we report the results from a cross-sectional study on nasal S. aureus/MRSA colonization of butchers, meat sellers, and cooks in Germany. We sampled 286 butchers and meat sellers in 26 butcheries and 319 cooks handling meat in 16 professional canteen kitchens. Swabs were processed on both blood agar plates and MRSA-selective plates. MRSA were confirmed by PCR for mec genes and by broth microdilution. All isolates were subjected to molecular typing. PCR for markers useful to differentiate human-adapted and animal-adapted subpopulations was performed due to the presence of clonal complexes known to occur in both livestock and humans (CC5, CC7, CC8, CC9, and CC398). Only two participants (0.33%) were colonized by MRSA (Hospital-associated MRSA ST22). Nasal colonization by methicillin-susceptible S. aureus (MSSA) was detected in 16.6% of cooks and in 26.2% of butchers and meat sellers. Among 16 of the isolates attributed to CC7, three were negative for the immune evasion gene cluster, suggesting an animal origin. Isolates attributed to CC5, CC8, and CC398 were negative for markers typical of animal-adapted subpopulations. The occupational handling of raw meat and raw meat products was not associated with nasal colonization by LA-MRSA.
- Subjects :
- Adult
Male
Veterinary medicine
Staphylococcus aureus
Meat
Health, Toxicology and Mutagenesis
lcsh:Medicine
MRSA
Biology
Toxicology
medicine.disease_cause
Article
Agar plate
03 medical and health sciences
meat
Young Adult
Drug Resistance, Bacterial
medicine
Humans
ddc:610
Raw meat
Nasal colonization
030304 developmental biology
0303 health sciences
030306 microbiology
business.industry
Broth microdilution
lcsh:R
food and beverages
occupational exposure
biochemical phenomena, metabolism, and nutrition
Middle Aged
Methicillin-resistant Staphylococcus aureus
Nasal Mucosa
Livestock
Female
Occupational exposure
business
610 Medizin und Gesundheit
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....0fef66c2f0c01002c6e4f268735b3eb7