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Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability
- Source :
- Molecules, Molecules; Volume 21; Issue 6; Pages: 741, Molecules, Vol 21, Iss 6, p 741 (2016)
- Publication Year :
- 2016
- Publisher :
- MDPI, 2016.
-
Abstract
- The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable.
- Subjects :
- Materials science
Surface Properties
Sonication
Alkalinity
Pharmaceutical Science
Intestinal fluid
Article
Analytical Chemistry
lcsh:QD241-441
response surface methodology
chemistry.chemical_compound
0404 agricultural biotechnology
lcsh:Organic chemistry
lysozyme
nanoliposomes
optimization
stability
Drug Discovery
Response surface methodology
Physical and Theoretical Chemistry
Particle Size
Liposome
Chromatography
Organic Chemistry
04 agricultural and veterinary sciences
040401 food science
Cholesterol
chemistry
Chemistry (miscellaneous)
Liposomes
Phosphatidylcholines
Molecular Medicine
Nanoparticles
Muramidase
Treatment time
Particle size
Lysozyme
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 21
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....0fd3ca7c09df9d8f0a9477e959a1ccf6