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Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability

Authors :
Rongfa Guan
Wu Zhipan
Guozou Cao
Jianguo Gao
Fei Lyu
Mingqi Liu
Source :
Molecules, Molecules; Volume 21; Issue 6; Pages: 741, Molecules, Vol 21, Iss 6, p 741 (2016)
Publication Year :
2016
Publisher :
MDPI, 2016.

Abstract

The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable.

Details

Language :
English
ISSN :
14203049
Volume :
21
Issue :
6
Database :
OpenAIRE
Journal :
Molecules
Accession number :
edsair.doi.dedup.....0fd3ca7c09df9d8f0a9477e959a1ccf6