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Prediction of fetal Rh D and Rh CcEe phenotype from maternal plasma with real-time polymerase chain reaction
- Source :
- Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis. 27(3)
- Publication Year :
- 2003
-
Abstract
- Real-time PCR methods for the detection of RHD and the C, c, and E allele of RHCE were applied for the prediction of fetal Rh phenotype using maternal plasma. In one of 36 samples investigated the DNA extraction failed. When we tested the remaining 35 samples for Rh antigens which were absent on the mother's red cells, the fetal D-status was correctly determined in 26 of 27 cases (1 false negative). Fetal C was tested correctly in 23 samples, c was true positive in the only c-negative woman and the fetal E-status was correctly determined in 35 cases. In conclusion real-time PCR of maternal plasma is a non-invasive method to determine fetal RH genotype. However, more studies are required for routine applications because the method is not 100% sensitive.
- Subjects :
- Genotype
Rh genotype
030204 cardiovascular system & hematology
Biology
Rh Isoimmunization
Polymerase Chain Reaction
Sensitivity and Specificity
03 medical and health sciences
0302 clinical medicine
Antigen
Computer Systems
Predictive Value of Tests
Pregnancy
Prenatal Diagnosis
Humans
False Positive Reactions
Allele
Genotyping
False Negative Reactions
Alleles
Glycoproteins
Retrospective Studies
Fetus
030219 obstetrics & reproductive medicine
Rh-Hr Blood-Group System
Racial Groups
Infant, Newborn
Hematology
DNA
Molecular biology
Phenotype
DNA extraction
Fetomaternal Transfusion
3. Good health
Real-time polymerase chain reaction
Blood Grouping and Crossmatching
Female
Subjects
Details
- ISSN :
- 14730502
- Volume :
- 27
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
- Accession number :
- edsair.doi.dedup.....0fc81fcd0cac346890230b2cdf3ed71c