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Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs

Authors :
Giorgio Marrubini
Giovanni Cappelli
Donato Squillaci
Lucia Trevisani
Giulio Arcangeli
Gianluca Bartolucci
Nicola Mucci
Elisabetta Bucaletti
Camillo Melzi
Stefano Dugheri
Source :
Separations, Volume 8, Issue 9, Separations, Vol 8, Iss 150, p 150 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Growing attention on carcinogenicity and mutagenicity of antineoplastic drugs (ADs) from the International Agencies has led to the present strict safe handling and administration regulations. Accordingly, one of the most common ways to assess occupational exposure to these substances is to identify and quantify possible surface contamination inside hospital preparation and administration units. Thus, it is essential to develop a fast and high-throughput monitoring method capable of identifying a significant number of ADs. The present study reports developing a UHPLC–MS/MS analysis to screen 26 ADs surface contamination through wipe test sampling. A Cortecs UPLC T3 50 × 2.1 mm (1.6 µm) column was selected to perform the analysis, using the evaluations of previous studies and the Product Quality Research Institute (PQRI) database. The design of experiments (DoE) methodological approach was used to optimize the chromatographic conditions concerning the best separation between all ADs. The limits of quantification for the analytes were between the pg/mL and ng/mL orders, and the turnaround time was limited to about 15 min. The obtained accuracy was mostly between 90% and 110% for all the analytes, while the precision was under 10% and a low matrix effect was observed for said analytes. Only vindesine and docetaxel presented lower performances.

Details

ISSN :
22978739
Volume :
8
Database :
OpenAIRE
Journal :
Separations
Accession number :
edsair.doi.dedup.....0fbe3b24813deb4f0679aa40d0871bce
Full Text :
https://doi.org/10.3390/separations8090150