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Non-digestible oligosaccharides modulate intestinal immune activation and suppress cow's milk allergic symptoms
- Source :
- Pediatric Allergy and Immunology, 25(8), 747. Blackwell Munksgaard
- Publication Year :
- 2014
-
Abstract
- BACKGROUND: Cow's milk allergy is a common food allergy in childhood and no effective preventive or curative treatment is available. This study aimed at comparing single short-chain galacto- (scGOS), long-chain fructo- (lcFOS) or pectin-derived acidic oligosaccharides (pAOS) and/or mixtures of scGOS/lcFOS (GF) or scGOS/lcFOS/pAOS (GFA) to prevent or treat food allergy. METHODS: In the preventive protocol, C3H/HeOuJ mice were fed diets containing single oligosaccharides or mixtures GF or GFA throughout the study protocol. In the treatment protocol, GF or GFA was provided for 4 wk starting after the last sensitization. The allergic skin response and anaphylaxis scores were determined, after oral challenge whey-specific immunoglobulins were measured, and qPCR for T-cell markers and Foxp3 counts using immunohistochemistry were performed on the small intestine and colon. RESULTS: Only in the preventive setting, the GF or GFA mixture, but not the single oligosaccharides, reduced the allergic skin response and whey-IgG1 levels in whey-sensitized mice, compared to the control diet. Both GF and GFA increased the number of Foxp3+ cells in the proximal small intestine of whey - compared to sham-sensitized mice. Expression of Th2 and Th17 mRNA markers increased in the middle part of the small intestine of whey-sensitized mice, which was prevented by GF. By contrast, GFA enhanced Tbet (Th1), IL-10 and TGF-β mRNA expression compared to GF which was maintained in the distal small intestine and/or colon. CONCLUSIONS: Dietary supplementation with scGOS/lcFOS or scGOS/lcFOS/pAOS during sensitization, both effectively reduce allergic symptoms but differentially affect mucosal immune activation in whey-sensitized mice.
- Subjects :
- medicine.medical_specialty
Immunology
Oligosaccharides
Milk allergy
Complex Mixtures
regulatory T cells
Immunomodulation
Mice
Intestinal mucosa
prevention
T-Lymphocyte Subsets
Transforming Growth Factor beta
Food allergy
Internal medicine
medicine
cow’s milk allergy
Animals
Humans
Immunology and Allergy
Sensitization
Mice, Inbred C3H
biology
business.industry
non-digestible oligosaccharides
FOXP3
food and beverages
Forkhead Transcription Factors
Allergens
medicine.disease
Immunity, Innate
Small intestine
Interleukin-10
Milk
medicine.anatomical_structure
Endocrinology
intestinal mucosa
Dietary Supplements
Pediatrics, Perinatology and Child Health
biology.protein
Cattle
Digestion
Immunization
Milk Hypersensitivity
Antibody
business
Anaphylaxis
Subjects
Details
- Language :
- English
- ISSN :
- 09056157
- Database :
- OpenAIRE
- Journal :
- Pediatric Allergy and Immunology, 25(8), 747. Blackwell Munksgaard
- Accession number :
- edsair.doi.dedup.....0fb61e88ee5e3fd33eef7b724be7ba8b