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A SERS-colorimetric dual-mode aptasensor for the detection of cancer biomarker MUC1
- Source :
- Analytical and bioanalytical chemistry. 412(23)
- Publication Year :
- 2020
-
Abstract
- Human mucin-1 (MUC1) has attracted considerable attention owing to its overexpression in diverse malignancies. Here, for the rapid and efficient detection of MUC1, we present a SERS-colorimetric dual-mode aptasensor, by integrating SERS probes with magnetic separation, which has several distinctive advantages. Using such a dual-mode aptasensor, the colorimetric functionality is distinguishable by the naked eye, providing a fast and straightforward screening ability for the detection of MUC1. Moreover, SERS-based detection greatly improves the detection sensitivity, reaching a limit of detection of 0.1 U/mL. In addition, the combination of SERS and colorimetric method holds the advantages of these two techniques and thereby increases the reliability and efficiency of MUC1 detection. On the one hand, the magnetic nanobeads functionalized with MUC1-specific aptamer were utilized as an efficient capturing substrate for separating MUC1 from biological complex medium. On the other hand, the gold-silver core-shell nanoparticles modified with Raman reporters and the complementary sequences of MUC1 were used as the signal indicator, which could simultaneously report the SERS signal and colorimetric change. This strategy can achieve a good detection range and realize MUC1 analysis in real patients' samples. Thus, we anticipate that this kind of aptasensor would provide promising potential applications in the diagnosis and prognosis of cancers. Graphical abstract.
- Subjects :
- Materials science
Aptamer
Nanotechnology
02 engineering and technology
Spectrum Analysis, Raman
01 natural sciences
Biochemistry
Analytical Chemistry
Neoplasms
Biomarkers, Tumor
Humans
skin and connective tissue diseases
Colorimetry
neoplasms
Detection limit
010401 analytical chemistry
Mucin-1
Dual mode
Aptamers, Nucleotide
021001 nanoscience & nanotechnology
Prognosis
digestive system diseases
0104 chemical sciences
Biomarker
Magnetic nanoparticles
Naked eye
0210 nano-technology
Subjects
Details
- ISSN :
- 16182650
- Volume :
- 412
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Analytical and bioanalytical chemistry
- Accession number :
- edsair.doi.dedup.....0fa9528b9fb3bcd9a54e3e4fd3f50fc9