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Site-specific, TALENs-mediated transformation of Bombyx mori
- Source :
- Insect Biochemistry and Molecular Biology. 55:26-30
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Transposon-based genetic transformation has facilitated insect functional genomics and new strategies of pest management. However, there is a need for alternative, site-specific approaches to overcome limitations of random integration (and associated position-effects) and potential instability of inserted transgenes. Here we describe a transposon-free, site-specific genetic transformation system mediated by transcription activator-like effector nucleases (TALENs) in the silkworm, Bombyx mori, a lepidopteran model insect. We successfully established a site-specific transgenic system with comparable transformation efficiency to transposon-based genetic transformation through microinjection of TALENs mRNA targeting the BmBLOS2 locus and a linearizable donor plasmid encoding an expression cassette of the DsRed2 red fluorescent protein. This system provides a valuable approach for insect transgenesis and will enable future functional gene analysis and generate novel applications in agricultural and medical insect pest-management technologies.
- Subjects :
- Male
Transposable element
Transgene
Molecular Sequence Data
Biology
Biochemistry
Article
Medicinal and Biomolecular Chemistry
Transcription Activator-Like Effector Nucleases
Genetics
Animals
Homologous recombination
Molecular Biology
Transcription activator-like effector nuclease
Base Sequence
Effector
fungi
Bombyx mori
Gene Transfer Techniques
Bombyx
TALENs
Transformation (genetics)
Insect Science
Female
Biochemistry and Cell Biology
Expression cassette
Zoology
Entomology
Functional genomics
Transformation efficiency
Subjects
Details
- ISSN :
- 09651748
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Insect Biochemistry and Molecular Biology
- Accession number :
- edsair.doi.dedup.....0fa362a969d80aaf43283b07dc03f616