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Novel methods of genome shuffling in Saccharomyces cerevisiae
- Source :
- Biotechnology letters. 31(5)
- Publication Year :
- 2008
-
Abstract
- Genome shuffling can improve complex phenotypes; however, there are several obstacles towards its broader applicability due to increased complexity of eukaryotic cells. Here, we describe novel, efficient and reliable methods for genome shuffling to increase ethanol production of Saccharomyces cerevisiae. Using yeast sexual and asexual reproduction by itself, mutant diploid cells were shuffled through highly efficient sporulation and adequate cross among the haploid cells, followed by selection on the special plates. The selected strain obtained after three round genome shuffling not only distinctly improved the resistance to ethanol, but also, increased ethanol yield by up to 13% compared with the control.
- Subjects :
- Genetics
Ascomycota
biology
Ethanol
Mutant
Saccharomyces cerevisiae
Bioengineering
Asexual reproduction
DNA Shuffling
General Medicine
Haploidy
biology.organism_classification
Applied Microbiology and Biotechnology
Genome
Phenotype
Diploidy
Yeast
Genetic Enhancement
Ploidy
Genome, Fungal
Biotechnology
Subjects
Details
- ISSN :
- 15736776
- Volume :
- 31
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Biotechnology letters
- Accession number :
- edsair.doi.dedup.....0f9031953e332fbc06d38acd6f33cc0f