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Membrane Microvesicles as Actors in the Establishment of a Favorable Prostatic Tumoral Niche: A Role for Activated Fibroblasts and CX3CL1-CX3CR1 Axis
- Source :
- Cancer Research, Cancer Research, American Association for Cancer Research, 2009, 69 (3), pp.785-793. ⟨10.1158/0008-5472.CAN-08-1946⟩
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- Tumor microenvironment is enriched in plasma membrane microvesicles (MV) shed from all cell types that constitute the tumor mass, reflecting the antigenic profile of the cells they originate from. Fibroblasts and tumor cells mutually communicate within tumor microenvironment. Recent evidences suggest that tumor-derived MVs (TMV) exert a broad array of biological functions in cell-to-cell communication. To elucidate their role in cancer-to-fibroblast cell communication, TMV obtained from two prostate carcinoma cell lines with high and weak metastatic potential (PC3 and LnCaP, respectively) have been characterized. TMV exhibit matrix metalloproteinases (MMP) and extracellular MMP inducer at their surface, suggesting a role in extracellular matrix degradation. Moreover, TMV not only induce the activation of fibroblasts assessed through extracellular signal-regulated kinase 1/2 phosphorylation and MMP-9 up-regulation, increase motility and resistance to apoptosis but also promote MV shedding from activated fibroblasts able in turn to increase migration and invasion of highly metastatic PC3 cells but not LnCaP cells. PC3 cell chemotaxis seems, at least partially, dependent on membrane-bound CX3CL1/fractalkine ligand for chemokine receptor CX3CR1. The present results highlight a mechanism of mutual communication attributable not only to soluble factors but also to determinants harbored by MV, possibly contributing to the constitution of a favorable niche for cancer development. [Cancer Res 2009;69(3):785–93]
- Subjects :
- Male
Cancer Research
[SDV]Life Sciences [q-bio]
CX3C Chemokine Receptor 1
Cell Communication
Biology
Adenocarcinoma
03 medical and health sciences
CX3CR1
0302 clinical medicine
Cell Movement
Cell Line, Tumor
fibroblasts
LNCaP
Extracellular
medicine
Humans
Neoplasm Invasiveness
Phosphorylation
Fibroblast
030304 developmental biology
Mitogen-Activated Protein Kinase 1
Organelles
0303 health sciences
Cell chemotaxis
Tumor microenvironment
Mitogen-Activated Protein Kinase 3
Chemokine CX3CL1
Microvesicle
Cell Membrane
Prostatic Neoplasms
microenvironment
Microvesicles
Cell biology
tumoral
medicine.anatomical_structure
Oncology
Matrix Metalloproteinase 9
030220 oncology & carcinogenesis
Immunology
Receptors, Chemokine
Stromal Cells
Extracellular Matrix Degradation
microvesicles
Subjects
Details
- Language :
- English
- ISSN :
- 00085472 and 15387445
- Database :
- OpenAIRE
- Journal :
- Cancer Research, Cancer Research, American Association for Cancer Research, 2009, 69 (3), pp.785-793. ⟨10.1158/0008-5472.CAN-08-1946⟩
- Accession number :
- edsair.doi.dedup.....0f85e90d169bfe773f767c8fabbdc22c