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Trafficking of a Dual-Modality Magnetic Resonance and Fluorescence Imaging Superparamagnetic Iron Oxide-Based Nanoprobe to Lymph Nodes
- Source :
- Molecular Imaging and Biology. 13:1163-1172
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- This study aims to develop and characterize the trafficking of a dual-modal agent that identifies primary draining or sentinel lymph node (LN). Herein, a dual-reporting silica-coated iron oxide nanoparticle (SCION) is developed. Nude mice were imaged by magnetic resonance (MR) and optical imaging and axillary LNs were harvested for histological analysis. Trafficking through lymphatics was observed with intravital and ex vivo confocal microscopy of popliteal LNs in B6-albino, CD11c-EYFP, and lys-EGFP transgenic mice. In vivo, SCION allows visualization of LNs. The particle’s size and surface functionality play a role in its passive migration from the intradermal injection site and its minimal uptake by CD11c+ dendritic cells and CD169+ and lys+ macrophages. After injection, SCION passively migrates to LNs without macrophage uptake and then can be used to image LN(s) by MRI and fluorescence. Thus, SCION can potentially be developed for use in sentinel node resections or for intralymphatic drug delivery.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Fluorescence-lifetime imaging microscopy
Sentinel lymph node
Mice, Nude
Fluorescence
Article
law.invention
Mice
In vivo
Confocal microscopy
law
medicine
Animals
Radiology, Nuclear Medicine and imaging
Magnetite Nanoparticles
Lymph node
Microscopy, Confocal
Staining and Labeling
integumentary system
Chemistry
Dextrans
Sentinel node
Silicon Dioxide
Magnetic Resonance Imaging
medicine.anatomical_structure
Oncology
Nanoparticles
Lymph Nodes
Molecular imaging
Ex vivo
Subjects
Details
- ISSN :
- 18602002 and 15361632
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular Imaging and Biology
- Accession number :
- edsair.doi.dedup.....0f80fb780ad827b5abd25b1c118e979c