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Engineering of cell-laden gelatin-based microgels for cell delivery and immobilization in regenerative therapies
- Source :
- Clinical Hemorheology and Microcirculation. 67:251-259
- Publication Year :
- 2017
- Publisher :
- IOS Press, 2017.
-
Abstract
- Cell-based therapies often face the challenge of low cell retention and viability upon transplantation. Hence, biomaterials, which can immobilize transplanted cells, while at the same time support cell viability, are essential for successful clinical application. Noteworthy, biomaterials in the micrometer range such as microcapsules or microspheres have the advantage of a minimally invasive introduction into tissue.Hence, we established an approach to generate gelatin-based cell carriers in the form of microspherical hydrogels. Fibroblasts were microencapsulated in glycidylmethacrylate (GMA)-functionalized gelatin by photopolymerization. While the degree of GMA-functionalization was kept constant, the hydrogel cross-linking density was adjusted by varying the time of irradiation or the average gelatin-chain length.Stable microspheres were synthesized from 10 wt% GMA-gelatin solutions for all irradiation periods tested (0.5 -2 min). Evaluation of cell viability revealed that microgels with the same weight content of biopolymer but with decreased cross-linking densities and thus decreased storage and E modulus, resulted in best cell support. Noteworthy, encapsulated cells partially migrated out of the microspheres and attached to the spherical surface.10 wt% GMA-gelatin-based hydrogels with E moduli comparable to the native cellular niche proved to be a promising biomaterial suitable for the production of cell-laden microspheres and shall be evaluated further for biomedical application.
- Subjects :
- 0301 basic medicine
food.ingredient
Physiology
Cell
02 engineering and technology
engineering.material
Gelatin
Mice
03 medical and health sciences
food
Physiology (medical)
medicine
Animals
Regeneration
Viability assay
Cell encapsulation
Tissue Engineering
Chemistry
Biomaterial
Hydrogels
Hematology
021001 nanoscience & nanotechnology
Transplantation
030104 developmental biology
medicine.anatomical_structure
Self-healing hydrogels
engineering
Biopolymer
0210 nano-technology
Cardiology and Cardiovascular Medicine
Biomedical engineering
Subjects
Details
- ISSN :
- 18758622 and 13860291
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Clinical Hemorheology and Microcirculation
- Accession number :
- edsair.doi.dedup.....0f7e69a469a91fccefbcde675965d36a