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Maternal obesity is associated with phenotypic alterations in fetal immune cells by single-cell mass cytometry
- Source :
- Am J Reprod Immunol
- Publication Year :
- 2020
-
Abstract
- Problem Prenatal exposure to metabolic dysregulation arising from maternal obesity can have negative health consequences in post-natal life. To date, the specific effects of maternal obesity on fetal immunity at a cellular level have not been well characterized. Method of study Using cord blood mononuclear cells (CBMCs) and cord plasma (n = 9/group) isolated from infants born to women with a high body mass index (BMI>25kg/m2 ) compared to women with a normal BMI (18-25kg/m2 ), we evaluated differences in immune cell populations using single-cell mass cytometry (CyTOF). CBMCs were matched according to potentially confounding variables, such as maternal and gestational age, ethnicity, smoking status, and gravidity. Statistical results were adjusted for fetal sex. Data were analyzed by viSNE and FlowSOM softwares in Cytobank™ . Results In newborn CBMCs from women with high BMI, we observed changes in frequency and phenotype of immune cell populations, including significant increases in CD4+ T cells and decreases in myeloid cell populations. IL-12p40 and MDC concentrations were significantly elevated in the high BMI group compared to control. Conclusion This study demonstrates an association between maternal obesity and fetal immunity. Our results warrant following long-term immunologic outcomes and associated clinical risks in children born to women with a high pre-pregnancy BMI.
- Subjects :
- 0301 basic medicine
CD4-Positive T-Lymphocytes
Male
Risk
Myeloid
Immunology
Physiology
Mass Spectrometry
Article
Body Mass Index
Obesity, Maternal
03 medical and health sciences
0302 clinical medicine
Immune system
Pregnancy
medicine
Immunology and Allergy
Humans
Mass cytometry
Myeloid Cells
Cells, Cultured
Fetus
030219 obstetrics & reproductive medicine
business.industry
Interleukin-12 Subunit p40
Tumor Suppressor Proteins
Infant, Newborn
Obstetrics and Gynecology
Gestational age
medicine.disease
Fetal Blood
Obesity
Up-Regulation
ADAM Proteins
030104 developmental biology
medicine.anatomical_structure
Phenotype
Reproductive Medicine
Cord blood
Female
Single-Cell Analysis
business
Body mass index
Subjects
Details
- ISSN :
- 16000897
- Volume :
- 85
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Accession number :
- edsair.doi.dedup.....0f7444d5238dc8e4686701fa10c710cd