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The combined use of Bacillus subtilis-based probiotic and anticoccidial herb had a better anti-Eimeria tenella efficiency
- Source :
- Journal of Applied Poultry Research, Vol 30, Iss 3, Pp 100181-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- SUMMARY The objective of this work is to investigate the feed supplementation with Bacillus subtilis-based probiotic and methanol extraction of anticoccidial herbs (leaves of Artemisia annua Linn., Dichroa febrifuga Lour., and peel of Punica granatum L.) individually or in combination as alternatives during chicken E. tenella infection. A total of 264 chickens were divided into 11 groups (n = 8 × 3). They were control group (G1), E. tenella infected group (G2), E. tenella infected with diclazuril treated group (G3). G4 to G6 were E. tenella infected and treated with 300 mg/kg body weight of methanol extract of herb in feed (3 different kinds of herbs: leaves of Artemisia annua Linn., Dichroa febrifuga Lour., and peel of Punica granatum L.), respectively. G7 to G9 were infected and probiotic-herb combined groups: 5 × 108 cfu/kg B. subtilis was added to feed on the basis of G4 to G6, respectively. The remaining groups are both feed with B. subtilis at 5 × 108 cfu/kg in feed but infected (G10) and uninfected (G11). The anticoccidial efficacy was assessed by: bloody feces scores, lesion scores, relative weight gain rate, oocysts excretion, and mRNA expression levels (occludin, ZO-1, IL-17A and IL-2) in ceca. All the interventions reduced all the indexes, as compared to that of G2. The use of B. subtilis combined with herb significantly reduced the oocysts excretion and IL-17A mRNA level as compared to herb only groups. In summary, we conclude that the combination of B. subtilis and anticoccidial herb have potential development value.
- Subjects :
- 0301 basic medicine
food.ingredient
Artemisia annua
Bacillus subtilis
anticoccidial herb
complex mixtures
SF1-1100
Eimeria
Food processing and manufacture
law.invention
03 medical and health sciences
chemistry.chemical_compound
Probiotic
food
Diclazuril
law
biology
Traditional medicine
Dichroa febrifuga
030108 mycology & parasitology
TP368-456
biology.organism_classification
Animal culture
030104 developmental biology
chemistry
Herb
Punica
Animal Science and Zoology
Eimeria tenella
probiotic
Subjects
Details
- Language :
- English
- ISSN :
- 10566171
- Volume :
- 30
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Poultry Research
- Accession number :
- edsair.doi.dedup.....0f6f2df416503c600d66f03ac13e601a