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Molecular signature and in vivo behavior of bone marrow endosteal and subendosteal stromal cell populations and their relevance to hematopoiesis
- Source :
- Experimental cell research. 318(19)
- Publication Year :
- 2011
-
Abstract
- In the bone marrow cavity, hematopoietic stem cells (HSC) have been shown to reside in the endosteal and subendosteal perivascular niches, which play specific roles on HSC maintenance. Although cells with long-term ability to reconstitute full hematopoietic system can be isolated from both niches, several data support a heterogenous distribution regarding the cycling behavior of HSC. Whether this distinct behavior depends upon the role played by the stromal populations which distinctly create these two niches is a question that remains open. In the present report, we used our previously described in vivo assay to demonstrate that endosteal and subendosteal stromal populations are very distinct regarding skeletal lineage differentiation potential. This was further supported by a microarray-based analysis, which also demonstrated that these two stromal populations play distinct, albeit complementary, roles in HSC niche. Both stromal populations were preferentially isolated from the trabecular region and behave distinctly in vitro, as previously reported. Even though these two niches are organized in a very close range, in vivo assays and molecular analyses allowed us to identify endosteal stroma (F-OST) cells as fully committed osteoblasts and subendosteal stroma (F-RET) cells as uncommitted mesenchymal cells mainly represented by perivascular reticular cells expressing high levels of chemokine ligand, CXCL12. Interestingly, a number of cytokines and growth factors including interleukin-6 (IL-6), IL-7, IL-15, Hepatocyte growth factor (HGF) and stem cell factor (SCF) matrix metalloproteases (MMPs) were also found to be differentially expressed by F-OST and F-RET cells. Further microarray analyses indicated important mechanisms used by the two stromal compartments in order to create and coordinate the “quiescent” and “proliferative” niches in which hematopoietic stem cells and progenitors reside.
- Subjects :
- Male
Stromal cell
Cellular differentiation
Stem cell factor
Biology
Bone and Bones
Article
Mice
Bone Marrow
medicine
Animals
Progenitor cell
Stem Cell Niche
Cells, Cultured
Mice, Inbred BALB C
Stem Cell Factor
Hepatocyte Growth Factor
Gene Expression Profiling
Interleukins
Mesenchymal stem cell
Cell Differentiation
Cell Biology
Hematopoietic Stem Cells
Chemokine CXCL12
Cell biology
Hematopoiesis
Mice, Inbred C57BL
medicine.anatomical_structure
Immunology
Hepatocyte growth factor
Bone marrow
Stem cell
Stromal Cells
medicine.drug
Subjects
Details
- ISSN :
- 10902422
- Volume :
- 318
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....0f6503f188e3a4977c788777ce6dbdca