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Ectopic Expression of Col2.3 and Col3.6 Promoters in the Brain and Association with Leptin Signaling
- Source :
- Cells Tissues Organs. 194:268-273
- Publication Year :
- 2011
- Publisher :
- S. Karger AG, 2011.
-
Abstract
- The collagen 2.3 and 3.6 promoters have been used to drive Cre expression for generation of conditional transgenic mutant mice. Within the bone, Col3.6 is expressed by mesenchymal precursor cells and their downstream progeny, while Col2.3 is more osteoblast specific. Our generation of transgenic mice with Col2.3-Cre- and Col3.6-Cre-driven deletion of the long-form leptin receptor (ObRb) necessitated a thorough analysis of the nonspecific expression of these promoters in the central nervous system. Both Col2.3 and Col3.6 were capable of forcing loxP recombination in the brain as demonstrated by EGFP expression in ROSA reporter mice. Expression of Col2.3 was limited to the central base of the brain near the third ventricle. In contrast, robust expression of Col3.6 was noted throughout the brain, centering near the distal third ventricle, third ventricle, and aqueduct. We subsequently analyzed the colocalization of leptin-responsive P-Stat3 neurons with Col3.6-expressing neurons. Approximately 5–10% colocalization was noted in leptin-responsive brain areas such as the arcuate nucleus, dorsal medial hypothalamus, ventral premammillary nucleus, and lateral hypothalamus. Injection of 3.6Cre+F/F ObRb knockout mice with leptin confirmed the presence of an intact P-Stat3 response that was dampened in the lateral hypothalamus (p < 0.050). This test was done to explore the contribution of neural leptin signaling to the bone phenotype of the 3.6Cre+F/F mice. Our analysis indicates that neural ObRb deletion, while present, is likely not the sole driver of femoral changes through traditional sympathetic circuits.
- Subjects :
- Paper
Leptin
STAT3 Transcription Factor
Aging
medicine.medical_specialty
Histology
Lateral hypothalamus
Green Fluorescent Proteins
Central nervous system
Collagen Type VI
Biology
Mice
Arcuate nucleus
Internal medicine
medicine
Animals
Promoter Regions, Genetic
Collagen Type II
Neurons
Recombination, Genetic
Third ventricle
Leptin receptor
Integrases
Brain
Colocalization
Protein Transport
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
Ectopic expression
Anatomy
Signal Transduction
Subjects
Details
- ISSN :
- 14226421 and 14226405
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Cells Tissues Organs
- Accession number :
- edsair.doi.dedup.....0f570bcfd44841f6874ff49614038526