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The effect of empiric antimicrobial treatment duration on detection of bacterial DNA in sterile surgical specimens
- Source :
- PLoS ONE, Vol 12, Iss 2, p e0171074 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Initial antimicrobial treatment of patients with deep seated or invasive infections is typically empiric. Usually, cultures of specimens obtained from the suspected source of infection are performed to identify pathogens and guide continued antimicrobial treatment. When patients present with signs and symptoms of infection, but sterile body fluid or tissue specimens cannot be obtained in a timely fashion, growth of bacterial pathogens in culture may be inhibited following initiation of empiric antibiotic treatment. To address this clinical dilemma, we performed a prospective evaluation of conventional culture vs. PCR coupled to electrospray ionization mass spectrometry (PCR/ESI-MS) on sterile body fluids and tissues submitted to the diagnostic microbiology lab following initiation of empiric antibiotic treatment for patients with suspected infection. In this series of surgical samples, PCR/ESI-MS identified bacterial pathogen(s) in 56% (49/87) of patients with non-diagnostic cultures. Examination of patients stratified by antibiotic treatment duration demonstrated that PCR/ESI-MS sustains high rates of bacterial DNA detection over time by generalized estimating equation models (p
- Subjects :
- Male
Physiology
Treatment duration
Antibiotics
lcsh:Medicine
Pathology and Laboratory Medicine
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
Odds Ratio
Medicine and Health Sciences
030212 general & internal medicine
lcsh:Science
Pathogen
Polymerase chain reaction
High rate
Aged, 80 and over
Multidisciplinary
Antimicrobials
Drugs
Bacterial Infections
Middle Aged
Antimicrobial
3. Good health
Anti-Bacterial Agents
Bacterial Pathogens
Body Fluids
Treatment Outcome
Medical Microbiology
Female
Pathogens
Anatomy
Bacterial dna
Research Article
Adult
DNA, Bacterial
Spectrometry, Mass, Electrospray Ionization
Patients
medicine.drug_class
Surgical and Invasive Medical Procedures
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Young Adult
Microbial Control
medicine
Humans
Microbial Pathogens
Aged
Body fluid
Pharmacology
Inpatients
Bacteria
lcsh:R
Biology and Life Sciences
Bacterial Load
Health Care
Specimen Preparation and Treatment
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....0f5386ae321c0fbb6914996450648e39