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Effect of UVA-activated Riboflavin on Dentin Bonding
- Publication Year :
- 2011
- Publisher :
- SAGE Publications, 2011.
-
Abstract
- Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds. © 2011 International & American Associations for Dental Research.
- Subjects :
- nanoleakage
collagen type 1, cross linking reagent, dentin bonding agent, matrix metalloproteinase, riboflavin, XP Bond, XP-Bond
Matrix metalloproteinase inhibitor
Ultraviolet Rays
Riboflavin
Dentistry
Dental bonding
Matrix (biology)
stomatognathic system
Ultimate tensile strength
Dentin
medicine
riboflavin, dentin bonding agent, collagen cross-linking, matrix metalloproteinases, nanoleakage, dentin matrix
Humans
Proanthocyanidins
Vitis
General Dentistry
Grape Seed Extract
Chemistry
business.industry
Bond strength
Dental Bonding
matrix metalloproteinases
Research Reports
Resin Cements
stomatognathic diseases
medicine.anatomical_structure
Cross-Linking Reagents
Glutaral
Dentin-Bonding Agents
Electrophoresis, Polyacrylamide Gel
Adhesive
Collagen
dentin matrix
business
dentin bonding agent
collagen cross-linking
Tannins
Nuclear chemistry
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0f4544153cfec3076f4e60563d4577ef