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Identification of HSPA8 as an interacting partner of MAB21L2 and an important factor in eye development
- Source :
- Developmental dynamics : an official publication of the American Association of AnatomistsREFERENCES.
- Publication Year :
- 2022
-
Abstract
- Pathogenic variants in human MAB21L2 result in microphthalmia, anophthalmia, and coloboma. The exact molecular function of MAB21L2 is currently unknown. We conducted a series of yeast two-hybrid (Y2H) experiments to determine protein interactomes of normal human and zebrafish MAB21L2/mab21l2 as well as human disease-associated variant MAB21L2-p.(Arg51Gly) using human adult retina and zebrafish embryo libraries.These screens identified klhl31, tnpo1, TNPO2/tnpo2, KLC2/klc2, and SPTBN1/sptbn1 as co-factors of MAB21L2/mab21l2. Several factors, including hspa8 and hspa5, were found to interact with MAB21L2-p.Arg51Gly but not wild-type MAB21L2/mab21l2 in Y2H screens. Further analyses via 1-by-1 Y2H assays, co-immunoprecipitation, and mass spectrometry revealed that both normal and variant MAB21L2 interact with HSPA5 and HSPA8. In situ hybridization detected co-expression of hspa5 and hspa8 with mab21l2 during eye development in zebrafish. Examination of zebrafish mutant hspa8This study identifies heat shock proteins as interacting partners of MAB21L2/mab21l2 and suggests a role for this interaction in vertebrate eye development.
- Subjects :
- Developmental Biology
Subjects
Details
- ISSN :
- 10970177
- Database :
- OpenAIRE
- Journal :
- Developmental dynamics : an official publication of the American Association of AnatomistsREFERENCES
- Accession number :
- edsair.doi.dedup.....0f420503740dd5bca456bab05d86ca7f