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pI(Cln) and cytosolic F-actin constitute a heteromeric complex with a constant molecular mass in rat skeletal muscles
- Source :
- Journal of biochemistry. 126(4)
- Publication Year :
- 1999
-
Abstract
- To elucidate the function of pI(Cln), its localization in subcellular organellae was investigated. A specific polyclonal anti-pI(Cln) antibody detected the soluble 38-kDa pI(Cln) exclusively in the cytosols of rat heart, lung, liver, spleen, skeletal muscle, testis, and brain, but not rat kidney. pI(Cln)-associated proteins in skeletal muscle were also analyzed. Native-gradient PAGE showed a single 340-kDa protein band reactive to anti-pI(Cln) antibody. This band also stained with anti-actin antibody. Two-dimensional PAGE and immunoprecipitation analysis indicated that all of the pI(Cln) was present in association with actin of a constant length: the molecular ratio of pI(Cln) to actin was roughly 1:7. In addition, all actin in the cytosol fractions was found in association with pI(Cln). These results suggest the possibility that skeletal muscle pI(Cln) controls the length of cytosolic F-actin.
- Subjects :
- Male
medicine.medical_specialty
Immunoprecipitation
Macromolecular Substances
Molecular Sequence Data
Biology
Biochemistry
Ion Channels
Rats, Sprague-Dawley
Cytosol
Chloride Channels
Internal medicine
medicine
Pi
Animals
Tissue Distribution
Amino Acid Sequence
Muscle, Skeletal
Molecular Biology
Peptide sequence
Actin
Molecular mass
Skeletal muscle
General Medicine
Molecular biology
Actins
Rats
Molecular Weight
medicine.anatomical_structure
Endocrinology
Polyclonal antibodies
biology.protein
Subcellular Fractions
Subjects
Details
- ISSN :
- 0021924X
- Volume :
- 126
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of biochemistry
- Accession number :
- edsair.doi.dedup.....0f410c1c2f64142a4e1664d59e0fd4ad