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New Irradiation Method with Indocyanine Green-Loaded Nanospheres for Inactivating Periodontal Pathogens
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 18, Iss 1, p 154 (2017), International Journal of Molecular Sciences; Volume 18; Issue 1; Pages: 154
- Publication Year :
- 2016
-
Abstract
- Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjunctive strategy for periodontitis treatments. However, use of aPDT for periodontal treatment is complicated by the difficulty in accessing morphologically complex lesions such as furcation involvement, which the irradiation beam (which is targeted parallel to the tooth axis into the periodontal pocket) cannot access directly. The aim of this study was to validate a modified aPDT method that photosensitizes indocyanine green-loaded nanospheres through the gingivae from outside the pocket using a diode laser. To establish this trans-gingival irradiation method, we built an in vitro aPDT model using a substitution for gingivae. Irradiation conditions and the cooling method were optimized before the bactericidal effects on Porphyromonas gingivalis were investigated. The permeable energy through the gingival model at irradiation conditions of 2 W output power in a 50% duty cycle was comparable with the transmitted energy of conventional irradiation. Intermittent irradiation with air cooling limited the temperature increase in the gingival model to 2.75 °C. The aPDT group showed significant bactericidal effects, with reductions in colony-forming units of 99.99% after 5 min of irradiation. This effect of aPDT against a periodontal pathogen demonstrates the validity of trans-gingival irradiation for periodontal treatment.
- Subjects :
- 0301 basic medicine
Gingival and periodontal pocket
medicine.medical_treatment
Dentistry
Photodynamic therapy
lcsh:Chemistry
diode laser
chemistry.chemical_compound
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
Photosensitizing Agents
biology
Absorption, Radiation
General Medicine
Computer Science Applications
Anti-Bacterial Agents
Cold Temperature
photodynamic therapy
Lasers, Semiconductor
Porphyromonas gingivalis
Nanospheres
Indocyanine Green
030103 biophysics
Periodontal treatment
indocyanine green
Models, Biological
Catalysis
Permeability
Article
Periodontal pathogen
Inorganic Chemistry
03 medical and health sciences
medicine
Humans
Irradiation
Physical and Theoretical Chemistry
Periodontitis
Molecular Biology
Microbial Viability
business.industry
Organic Chemistry
030206 dentistry
biology.organism_classification
medicine.disease
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Photochemotherapy
business
Indocyanine green
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....0f3e35edd759b000ee31807a4d85b9cd