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The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine
The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine
- Source :
- Life science alliance. 6(1)
- Publication Year :
- 2022
-
Abstract
- The efficacy of the current influenza vaccines is frequently reduced because of antigenic drift, a trade-off of developing improved vaccines with broad cross-protective activity against influenza A viruses. In this study, we have successfully constructed a chimeric cytokine (CC) comprising the M2 protein, influenza A neuraminidase stalk, and interleukin-12. We produced virus-like particles (VLPs) containing CC and influenza hemagglutinin (HA) proteins using a baculovirus system in Eri silkworm pupae. The protective efficacy of the CCHA-VLP vaccine was evaluated in mice. The CCFkH5HA-VLP vaccine increased the survival rates of BALB/c mice, infected with a lethal dose of PRH1 and HKH5 viruses, to 80% and 100%, respectively. The results suggested that CCHA-VLP successfully induced potent cross-reactive protective immunity against infection with homologous and heterologous subtypes of the influenza A virus. This is the first study to design a CC-containing HA-VLP vaccine and validate its protective efficacy.
- Subjects :
- Mice, Inbred BALB C
Ecology
Health, Toxicology and Mutagenesis
Plant Science
Antibodies, Viral
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Mice
Hemagglutinins
Orthomyxoviridae Infections
Influenza Vaccines
Influenza, Human
Animals
Humans
Cytokines
Vaccines, Virus-Like Particle
Subjects
Details
- ISSN :
- 25751077
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Life science alliance
- Accession number :
- edsair.doi.dedup.....0f2f5e7b010a630d9dc102651cb031d6