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The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine

The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine

Authors :
Kuniaki Nerome
Toshifumi Imagawa
Shigeo Sugita
Youta Arasaki
Kenichi Maegawa
Kazunori Kawasaki
Tsuyoshi Tanaka
Shinji Watanabe
Hidekazu Nishimura
Tetsuro Suzuki
Kazumichi Kuroda
Isao Kosugi
Zenta Kajiura
Source :
Life science alliance. 6(1)
Publication Year :
2022

Abstract

The efficacy of the current influenza vaccines is frequently reduced because of antigenic drift, a trade-off of developing improved vaccines with broad cross-protective activity against influenza A viruses. In this study, we have successfully constructed a chimeric cytokine (CC) comprising the M2 protein, influenza A neuraminidase stalk, and interleukin-12. We produced virus-like particles (VLPs) containing CC and influenza hemagglutinin (HA) proteins using a baculovirus system in Eri silkworm pupae. The protective efficacy of the CCHA-VLP vaccine was evaluated in mice. The CCFkH5HA-VLP vaccine increased the survival rates of BALB/c mice, infected with a lethal dose of PRH1 and HKH5 viruses, to 80% and 100%, respectively. The results suggested that CCHA-VLP successfully induced potent cross-reactive protective immunity against infection with homologous and heterologous subtypes of the influenza A virus. This is the first study to design a CC-containing HA-VLP vaccine and validate its protective efficacy.

Details

ISSN :
25751077
Volume :
6
Issue :
1
Database :
OpenAIRE
Journal :
Life science alliance
Accession number :
edsair.doi.dedup.....0f2f5e7b010a630d9dc102651cb031d6