Back to Search
Start Over
Strategies for the screening of antibiotic residues in eggs: comparison of the validation of the classical microbiological method with an immunobiosensor method
- Source :
- Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Taylor & Francis, 2017, pp.1-19. ⟨10.1080/19440049.2017.1339331⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Efficient screening methods are needed to control antibiotic residues in eggs. A microbiological kit (Explorer® 2.0 test (Zeu Inmunotech, Spain)) and an immunobiosensor kit (Microarray II (AM® II) on Evidence Investigator™ system (Randox, UK)) have been evaluated and validated for screening of antibiotic residues in eggs, according to the European decision EC/2002/657 and to the European guideline for the validation of screening methods. The e-reader™ system, a new automatic incubator/reading system, was coupled to the Explorer 2.0 test. The AM II kit can detect residues of six different families of antibiotics in different matrices including eggs. For both tests, a different liquid/liquid extraction of eggs had to be developed. Specificities of the Explorer 2.0 and AM II kit were equal to 8% and 0% respectively. The detection capabilities were determined for 19 antibiotics, with representatives from different families, for Explorer 2.0 and 12 antibiotics for the AM II kit. For the nine antibiotics having a maximum residue limit (MRL) in eggs, the detection capabilities CCβ of Explorer 2.0 were below the MRL for four antibiotics, equal to the MRL for two antibiotics and between 1 and 1.5 MRLs for the three remaining antibiotics (tetracyclines). For the antibiotics from other families, the detection capabilities were low for beta-lactams and sulfonamides and satisfactory for dihydrostreptomycin (DHS) and fluoroquinolones, which are usually difficult to detect with microbiological tests. The CCβ values of the AM II kit were much lower than the respective MRLs for three detected antibiotics (tetracycline, oxytetracycline, tylosin). Concerning the nine other antibiotics, the detection capabilities determined were low. The highest CCβ was obtained for streptomycin (100 µg kg–1).
- Subjects :
- Veterinary medicine
Maximum Residue Limit
test immunologique
analysis
Health, Toxicology and Mutagenesis
Eggs
Antibiotics
analyse
veterinary drug
Toxicology
01 natural sciences
antibiotique
chemistry.chemical_compound
residues
antibiotic
Veterinary drug
résidus
04 agricultural and veterinary sciences
General Medicine
040401 food science
immunological test
3. Good health
Anti-Bacterial Agents
Streptomycin
medicine.drug
médicament vétérinaire
Tetracycline
medicine.drug_class
test microbiologique
Food Contamination
Oxytetracycline
Biology
Tylosin
biosensor
0404 agricultural biotechnology
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
medicine
Dihydrostreptomycin
microbiological test
business.industry
010401 analytical chemistry
Public Health, Environmental and Occupational Health
General Chemistry
Drug Residues
0104 chemical sciences
Biotechnology
chemistry
Spain
oeuf
egg
business
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 19440049 and 19440057
- Database :
- OpenAIRE
- Journal :
- Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, Taylor & Francis, 2017, pp.1-19. ⟨10.1080/19440049.2017.1339331⟩
- Accession number :
- edsair.doi.dedup.....0f1a5870f98925a223400eebab25713f