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Development and comparison of two dispersive liquid-liquid microextraction techniques coupled to high performance liquid chromatography for the rapid analysis of bisphenol A in edible oils
- Source :
- Journal of chromatography. A. 1295
- Publication Year :
- 2013
-
Abstract
- In this study, two novel sample extraction methods for the analysis of bisphenol A (BPA) in edible oils were developed by using liquid–liquid extraction followed by a dispersive liquid–liquid microextraction (LLE-DLLME) and reversed-phase dispersive liquid–liquid microextraction (RP-DLLME). RP-DLLME showed a superior characteristic over LLE-DLLME and other previously reported procedures because of its easy operation, short extraction time, high sensitivity, low organic solvent consumption and waste generation. The optimized extraction conditions of RP-DLLME for 1.0 g of edible oil diluted in 4 mL of n-hexane were: extractant, 100 μL 0.2 M sodium hydroxide solution (80% methanol, v/v); extraction time, 1 min; centrifugation, 3 min. The determination of BPA was carried out by high performance liquid chromatography coupled with a DAD detector. The method offered excellent linearity over a range of 0.010–0.5 μg g −1 with a correlation coefficient of r > 0.997. Intra-day and inter-day repeatability values expressed as relative standard deviation were 1.9% and 5.9%, respectively. The quantitation limit and detection limit were 6.3 and 2.5 ng g −1 . The target analyte was detected in 5 out of 16 edible oil samples. The recovery rates in real samples ranged from 89.5 to 99.7%.
- Subjects :
- Detection limit
Bisphenol A
Analyte
Chromatography
Liquid Phase Microextraction
Organic Chemistry
Extraction (chemistry)
Analytical chemistry
Food Contamination
General Medicine
Repeatability
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
chemistry.chemical_compound
chemistry
Phenols
Sodium hydroxide
Limit of Detection
Plant Oils
Methanol
Benzhydryl Compounds
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 18733778
- Volume :
- 1295
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....0efd401101c331f833d79ecf036ff2f9