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Characterization of phage Ï O139, a Vibrio cholerae O139 temperate bacteriophage with cohesive DNA termini
- Source :
- FEMS Microbiology Letters. 131:69-74
- Publication Year :
- 1995
- Publisher :
- Oxford University Press (OUP), 1995.
-
Abstract
- A temperate bacteriophage isolated from Vibrio cholerae O139, the new epidemic strain of cholera, was found to have a polyhedral head 65 nm in diameter and a rigid contractile tail 120 nm in length. The phage chromosome was a double-stranded DNA of 35 kb, with unique cohesive ends and had a G + C content of 58.8%. A restriction map of the phage DNA was constructed using the restriction endonucleases AvaI and BstEII. The phage, whose presence could be detected in nine out of 13 V. cholerae O139 isolates tested, was found to have identical chromosomal integration sites in all the strains. The phage attachment site (attP) was found to be located very close to one end of the genome.
- Subjects :
- viruses
Restriction Mapping
medicine.disease_cause
Microbiology
Bacteriophage
chemistry.chemical_compound
Restriction map
Vibrionaceae
Genetics
medicine
Bacteriophages
Lysogeny
Vibrio cholerae
Molecular Biology
Genomic organization
biology
Chromosome Mapping
biology.organism_classification
humanities
Temperateness
Blotting, Southern
Microscopy, Electron
Restriction enzyme
chemistry
DNA, Viral
DNA
Subjects
Details
- ISSN :
- 15746968 and 03781097
- Volume :
- 131
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....0ef6f313e760021ae42ab9198a16f14e