Additional file 8: of South African HIV-1 subtype C transmitted variants with a specific V2 motif show higher dependence on α4β7 for replication

The impact of HIV glycans on α4β7 reactivity. (A) Correlations between the glycan density, the length of V1/V2, V3, C3/V4, V5 and dependence on α4β7 (Act-1) for replication of 60 IEC. Blocks shaded in grey are those which were significant in a linear mixed model corrected for repeated measures. Beta coefficients and p values are shown below the respective blocks. (B) Frequency of predicted N-linked glycans in the conserved regions of gp120 relative to the percentage of viruses that showed high (green, n = 33) or low (blue, n = 27) dependency on α4β7. Significance was determined by the Fisher exact test where PNG234 (**p = 0.009) and PNG334 (**p = 0.006) were more frequent in viruses with high α4β7 dependence andPNG332 was more frequent in viruses with low α4β7 dependence(*p = 0.026).