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Defective sperm head decondensation undermines the success of ICSI in the bovine
- Publication Year :
- 2017
-
Abstract
- The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It is unknown whether defective oocyte activation and/or sperm head decondensation limit the success of this technique in this species. To elucidate where the main obstacle lies, we used homologous and heterologous ICSI and parthenogenetic activation procedures. We also evaluated whetherin vitromaturation negatively impacted the early stages of activation after ICSI. Here we showed that injected bovine sperm are resistant to nuclear decondensation by bovine oocytes and this is only partly overcome by exogenous activation. Remarkably, when we used heterologous ICSI,in vivo-matured mouse eggs were capable of mounting calcium oscillations and displaying normal PN formation following injection of bovine sperm, althoughin vitro-matured mouse oocytes were unable to do so. Together, our data demonstrate that bovine sperm are especially resistant to nuclear decondensation byin vitro-matured oocytes and this deficiency cannot be simply overcome by exogenous activation protocols, even by inducing physiological calcium oscillations. Therefore, the inability of a suboptimal ooplasmic environment to induce sperm head decondensation limits the success of ICSI in the bovine. Studies aimed to improve the cytoplasmic milieu ofin vitro-matured oocytes and to replicate the molecular changes associated within vivocapacitation and acrosome reaction will deepen our understanding of the mechanism of fertilization and improve the success of ICSI in this species.
- Subjects :
- 0301 basic medicine
Male
Embryology
medicine.medical_treatment
Acrosome reaction
Parthenogenesis
Heterologous
Cattle Diseases
Biology
Intracytoplasmic sperm injection
Article
Andrology
Embryo Culture Techniques
03 medical and health sciences
Mice
Endocrinology
Human fertilization
Species Specificity
Capacitation
medicine
Animals
Calcium Signaling
Sperm Injections, Intracytoplasmic
reproductive and urinary physiology
Cells, Cultured
Infertility, Male
Cell Nucleus
Sperm-Ovum Interactions
urogenital system
Obstetrics and Gynecology
Oocyte activation
Cell Biology
Chromatin Assembly and Disassembly
In vitro maturation
In Vitro Oocyte Maturation Techniques
030104 developmental biology
Reproductive Medicine
Sperm Head
Cattle
Female
Sperm Capacitation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0eec2daf6051b9a730f42aefa0b717c7