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A qPCR assay that specifically quantifies Tricholoma matsutake biomass in natural soil
- Source :
- Mycorrhiza. 26:847-861
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Tricholoma matsutake is an ectomycorrhizal basidiomycete that produces prized, yet uncultivable, "matsutake" mushrooms along densely developed mycelia, called "shiro," in the rhizosphere of coniferous forests. Pinus densiflora is a major host of this fungus in Japan. Measuring T. matsutake biomass in soil allows us to determine the kinetics of fungal growth before and after fruiting, which is useful for analyzing the conditions of the shiro and its surrounding mycorrhizosphere, predicting fruiting timing, and managing forests to obtain better crop yields. Here, we document a novel method to quantify T. matsutake mycelia in soil by quantifying a single-copy DNA element that is uniquely conserved within T. matsutake but is absent from other fungal species, including close relatives and a wide range of ectomycorrhizal associates of P. densiflora. The targeted DNA region was amplified quantitatively in cultured mycelia that were mixed with other fungal species and soil, as well as in an in vitro co-culture system with P. densiflora seedlings. Using this method, we quantified T. matsutake mycelia not only from shiro in natural environments but also from the surrounding soil in which T. matsutake mycelia could not be observed by visual examination or distinguished by other means. It was demonstrated that the core of the shiro and its underlying area in the B horizon are predominantly composed of fungal mycelia. The fungal mass in the A or A0 horizon was much lower, although many white mycelia were observed at the A horizon. Additionally, the rhizospheric fungal biomass peaked during the fruiting season.
- Subjects :
- Genetic Markers
0106 biological sciences
0301 basic medicine
Mycorrhizosphere
Plant Science
Biology
Polymerase Chain Reaction
Sensitivity and Specificity
01 natural sciences
03 medical and health sciences
Pinus densiflora
Species Specificity
Botany
Genetics
Biomass
DNA, Fungal
Molecular Biology
Soil Microbiology
Ecology, Evolution, Behavior and Systematics
Mycelium
Rhizosphere
Serine Endopeptidases
Tricholoma
fungi
General Medicine
Matsutake
equipment and supplies
biology.organism_classification
Kinetics
030104 developmental biology
Soil horizon
Genome, Fungal
Soil microbiology
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14321890 and 09406360
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Mycorrhiza
- Accession number :
- edsair.doi.dedup.....0ee17c55e3923f10cd22f4717c186245