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No predictive value of cytotoxic or helper T-cell precursor frequencies for outcome when analyzed from the graft after stem cell transplantation
- Source :
- Annals of hematology. 83(9)
- Publication Year :
- 2004
-
Abstract
- The predictive value of limiting dilution analyses (LDA) measuring cytotoxic and helper T-lymphocyte precursor (CTLp and HTLp) frequencies for outcome after stem cell transplantation (SCT) is still a matter of debate. One reason may be that CTLp and HTLp frequencies are determined in peripheral blood mononuclear cells (PBMC) and this responder cell population does not reflect the cell type composition of the graft. We assessed whether CTLp and HTLp LDA can predict complications after human leukocyte antigen (HLA)-identical SCT when CTLp and HTLp frequencies are analyzed in PBMC of the respective stem cell graft [bone marrow (BMMC) or granulocyte colony-stimulating factor (G-CSF)-mobilized PBMC] and compared to PBMC of PB. Host-specific CTLp frequencies measured in 25 patients and HTLp frequencies analyzed in 6 patients were low in all responder cell sources. CTLp and HTLp frequencies seen against HLA-mismatched unrelated third-party cells were high, but third-party-specific CTLp and HTLp frequencies were lower in G-CSF-PBMC than in PBMC ( p=0.047 for CTLp frequencies). Host-specific CTLp frequencies analyzed in all responder cell sources did not predict acute or chronic graft-versus-host disease (GVHD). Lower CTLp frequencies were detected in all responder cell sources from patients who relapsed after SCT than in patients without relapse, but the differences between both groups were statistically significant only in PBMC. In conclusion, a significant correlation was detected only between relapse and CTLp frequencies measured in PBMC. The lower frequency of third-party-specific cells in G-CSF-PBMC indicates that the mobilization procedure with G-CSF itself may influence results.
- Subjects :
- Adult
T cell
Population
Graft vs Host Disease
Human leukocyte antigen
Biology
Peripheral blood mononuclear cell
Predictive Value of Tests
Recurrence
medicine
Cytotoxic T cell
Humans
education
Cells, Cultured
education.field_of_study
Stem Cells
Cell Differentiation
Hematology
General Medicine
T-Lymphocytes, Helper-Inducer
Transplantation
medicine.anatomical_structure
Treatment Outcome
Immunology
Acute Disease
Chronic Disease
Bone marrow
Stem cell
Stem Cell Transplantation
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 09395555
- Volume :
- 83
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Annals of hematology
- Accession number :
- edsair.doi.dedup.....0ebf676cfb30ce57c8a397aa0fde6cb5