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Distribution of Myocilin and Extracellular Matrix Components in the Corneoscleral Meshwork of Human Eyes
- Source :
- Investigative Opthalmology & Visual Science. 44:4772
- Publication Year :
- 2003
- Publisher :
- Association for Research in Vision and Ophthalmology (ARVO), 2003.
-
Abstract
- Purpose To examine ultrastructurally the composition of major extracellular matrix (ECM) components and the distribution of myocilin in the trabecular lamellae of corneoscleral (CS) meshwork in normal human eyes. The codistribution of myocilin with ECM components was also investigated. Methods Postembedding immunoelectron microscopic studies were performed with antibodies against myocilin and other ECM components, including fibronectin, laminin, vitronectin, tenascin, elastin, fibrillin-1, microfibril-associated glycoprotein (MAGP)-1, decorin, versican, hyaluronic acid, and five types of collagen (I, III, IV, V, and VI). Double labeling of myocilin with other ECM components was performed with different sized gold particles. Results In the trabecular beams of CS meshwork, fibronectin, laminin, and collagen type IV were associated with basement membranes, whereas elastin was specifically localized to the core of elastic-like fibers. Several types of collagens, glycoproteins, proteoglycans, and hyaluronic acid were detected both in the collagen fibers and ground substances. Myocilin predominantly localized in the long-spacing collagens and sheath materials surrounding elastic-like fibers, codistributed with fibronectin, fibrillin-1, MAGP-1, decorin, and type VI collagen. Conclusions This study illustrated the composition of ECM materials in the trabecular lamellae of CS meshwork. Myocilin was specifically localized to long-spacing collagens and the surrounding sheath of elastic-like fibers interacting with microfibril-associated elements where changes have been documented to occur in glaucomatous and aging eyes.
- Subjects :
- Adult
genetic structures
Decorin
Tenascin
Basement Membrane
Cornea
Extracellular matrix
Trabecular Meshwork
Laminin
Humans
Eye Proteins
Microscopy, Immunoelectron
Myocilin
Aged
Glycoproteins
Extracellular Matrix Proteins
biology
Chemistry
Middle Aged
eye diseases
Cell biology
carbohydrates (lipids)
Fibronectin
Cytoskeletal Proteins
Biochemistry
biology.protein
Versican
sense organs
Fibrillin
Sclera
Subjects
Details
- ISSN :
- 15525783
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Investigative Opthalmology & Visual Science
- Accession number :
- edsair.doi.dedup.....0ea5caee877336f2f66ef5b432f4f98c