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Uc.416 + A promotes epithelial-to-mesenchymal transition through miR-153 in renal cell carcinoma
- Source :
- BMC Cancer, Vol 18, Iss 1, Pp 1-10 (2018), BMC Cancer
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background The transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers. However, the expression and biological role of T-UCRs in renal cell carcinoma (RCC) remain poorly understood. This study aimed to examine the expression and functional role of Uc.416 + A and analyze the association between Uc.416 + A and epithelial-to-mesenchymal transition in RCC. Methods Expression of Uc.416 + A in 35 RCC tissues, corresponding normal kidney tissues and 13 types of normal tissue samples was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We performed a cell growth and migration assay in RCC cell line 786-O transfected with negative control and siRNA for Uc.416 + A. We evaluated the relation between Uc.416 + A and miR-153, which has a complimentary site of Uc.416 + A. Results qRT-PCR analysis revealed that the expression of Uc.416 + A was higher in RCC tissues than that in corresponding normal kidney tissues. Inhibition of Uc.416 + A reduced cell growth and cell migration activity. There was an inverse correlation between Uc.416 + A and miR-153. Western blot analysis showed Uc.416 + A modulated E-cadherin, vimentin and snail. The expression of Uc.416 + A was positively associated with the expression of SNAI1, VIM and inversely associated with the expression of CDH1. Conclusions The expression of Uc.416 + A was upregulated in RCC and especially in RCC tissues with sarcomatoid change. Uc.416 + A promoted epithelial-to-mesenchymal transition through miR-153. These results suggest that Uc.416 + A may be a promising therapeutic target. Electronic supplementary material The online version of this article (10.1186/s12885-018-4863-y) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Cancer Research
Epithelial-Mesenchymal Transition
RNA, Untranslated
Uc.416 + A
Vimentin
medicine.disease_cause
lcsh:RC254-282
miR-153
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Genetics
medicine
Humans
Epithelial–mesenchymal transition
Carcinoma, Renal Cell
Cell Proliferation
biology
Reverse Transcriptase Polymerase Chain Reaction
Cell growth
Chemistry
Cell migration
Sarcomatoid change
Transfection
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Molecular biology
Kidney Neoplasms
Renal cell carcinoma
MicroRNAs
030104 developmental biology
Epithelial-to-mesenchymal transition
Oncology
Cell culture
030220 oncology & carcinogenesis
SNAI1
biology.protein
Carcinogenesis
Research Article
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....0e9db3900d0e0c538030818f4f0c4878