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Early commitment of naïve human CD4(+) T cells to the T follicular helper (T(FH)) cell lineage is induced by IL-12
- Source :
- Immunology and cell biology. 87(8)
- Publication Year :
- 2009
-
Abstract
- T follicular helper (T(FH)) cells are a specialized subset of CD4(+) T cells that localize to B-cell follicles, where they are positioned to provide help for the induction of optimal humoral immune responses. Key features of T(FH) cells are the expressions of CXCR5, ICOS, interleukin (IL)-21 and BCL-6. The requirements for human T(FH) cell development are unknown. Here we show that IL-6, IL-12, IL-21 and IL-23 are capable of inducing IL-21 expression in naïve CD4(+) T cells isolated from human tonsils, peripheral blood and cord blood. However, only IL-12 induced sustained expressions of CXCR5 and ICOS on these activated naïve CD4(+) T cells, and endowed them with the ability to provide increased help to B cells for their differentiation into immunoglobulin-secreting cells. The effects of IL-12 were independent of interferon-gamma and T-bet, and associated with upregulation of BCL-6 expression. Thus, these cytokines, particularly IL-12, are likely to act at an early stage during dendritic cell-mediated priming of naïve CD4(+) T cells into a T(FH) cell fate, and thus underpin antibody-mediated immunity.
- Subjects :
- CD4-Positive T-Lymphocytes
endocrine system
Lymphoid Tissue
Immunology
Biology
CXCR5
Interleukin 21
Immune system
Follicular phase
Immunology and Allergy
Cytotoxic T cell
Humans
Cell Lineage
Cells, Cultured
T follicular helper cell differentiation
Interleukins
Cell Differentiation
Cell Biology
Dendritic Cells
T-Lymphocytes, Helper-Inducer
Natural killer T cell
Interleukin-12
Coculture Techniques
Immunity, Innate
Phenotype
Interleukin 12
Subjects
Details
- ISSN :
- 14401711
- Volume :
- 87
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Immunology and cell biology
- Accession number :
- edsair.doi.dedup.....0e77c76a92e9c467e6d12e5da4a60ebb