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Protective effect against focal cerebral ischemia injury in acute phase of a novel invasive device for regional hypothermia
- Source :
- Journal of the Chinese Medical Association, Vol 78, Iss 1, Pp 67-75 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Background Systemic hypothermia is considered beneficial to stroke patients. However, many complications ensue. The aim of this study was to evaluate the effect of a new invasive regional cooling device in cerebral ischemic injury in a rat model. Methods After a pilot study confirming the efficiency of the cooling device, 15 adult male Sprague–Dawley rats, weighing 300–400 g, were randomly assigned into three groups: cooling device applied at 14°C and at 26°C, and a sham group. Focal cerebral ischemic injury was achieved by electrocauterization of the left middle cerebral artery through craniectomy and temporal occlusion of both common carotid arteries for 3 hours. Within 30 minutes after the end of ischemic injury, the cooling device was inserted into the rat brain through a stereotactic frame to provide regional hypothermia for 2 hours. The rats were sacrificed immediately after the 2-hour regional hypothermia. Results Although triphenyltetrazolium chloride staining showed smaller ischemic lesions in both the 26°C and 14°C groups compared to the control group, Fluoro Jade C staining showed no neuroprotective effects in the rostrum cerebral cortex in both groups. However, both triphenyltetrazolium chloride and Fluoro Jade C staining indicated significant beneficial effects in the caudal cerebral cortex in rats with cooling device applied at 26°C compared to the 14°C and control groups. Conclusion Our findings indicated that the device can effectively achieve regional hypothermia and could be beneficial for patients with cerebral ischemia during the acute phase.
- Subjects :
- Male
middle cerebral artery infarction
Ischemia
Neuroprotection
Brain ischemia
Rats, Sprague-Dawley
Hypothermia, Induced
Occlusion
medicine
Animals
Stroke
Medicine(all)
lcsh:R5-920
business.industry
General Medicine
Hypothermia
medicine.disease
stroke
brain ischemia
Rats
medicine.anatomical_structure
Cerebral cortex
Anesthesia
Electrocauterization
medicine.symptom
business
hypothermia
lcsh:Medicine (General)
Subjects
Details
- ISSN :
- 17264901
- Volume :
- 78
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of the Chinese Medical Association
- Accession number :
- edsair.doi.dedup.....0e52144c931baf722f0293594276e8dd
- Full Text :
- https://doi.org/10.1016/j.jcma.2014.07.009