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A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant staphylococcus aureus pandemic
- Source :
- Holden, M T G, Hsu, L-Y, Kurt, K, Weinert, L A, Mather, A E, Harris, S R, Strommenger, B, Layer, F, Witte, W, de Lencastre, H, Skov, R, Westh, H, Zemlickova, H, Coombs, G, Kearns, A M, Hill, R L R, Edgeworth, J, Gould, I, Gant, V, Cooke, J, Edwards, G F, McAdam, P, Templeton, K E, McCann, A, Zhou, Z, Castillo-Ramirez, S, Feil, E J, Hudson, L O, Enright, M C, Balloux, F, Aanensen, D M, Spratt, B G, Fitzgerald, J R, Parkhill, J, Achtman, M, Bentley, S D & Nübel, U 2013, ' A genomic portrait of the emergence, evolution, and global spread of a methicillin resistant Staphylococcus aureus pandemic ', Genome Research, vol. 23, no. 4, pp. 653-664 . https://doi.org/10.1101/gr.147710.112, Genome Research
- Publication Year :
- 2013
- Publisher :
- Cold Spring Harbor Laboratory Press, 2013.
-
Abstract
- The widespread use of antibiotics in association with high-density clinical care has driven the emergence of drug-resistant bacteria that are adapted to thrive in hospitalised patients. Of particular concern are globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones that cause outbreaks and epidemics associated with healthcare. The most rapidly spreading and tenacious healthcare-associated clone in Europe currently is EMRSA-15, a lineage that was first detected in the UK in the early 1990s and subsequently spread throughout Europe and beyond. To understand the genetic events that have accompanied the emergence of the EMRSA-15 pandemic, we obtained genome sequences for 193 isolates that were chosen for their geographical and temporal diversity, and belong to the same multilocus sequence type as EMRSA-15. Using phylogenomic methods, we were able to show that the current pandemic population of EMRSA-15 descends from a healthcare-associated MRSA epidemic that spread through England in the 1980s, which had itself previously emerged from a primarily community-associated methicillin-sensitive population. The emergence of fluoroquinolone resistance in this EMRSA-15 sub-clone in the English Midlands during the mid-1980s appears to have played a key role in triggering pandemic spread, and occurred shortly after the first clinical trials of this drug. Genome-based coalescence analysis estimated that the population of this sub-clone over the last twenty years has grown four times faster than its progenitor. Using comparative genomic analysis we were able to identify the molecular genetic basis of 99.8% of the antimicrobial resistance phenotypes of the isolates, highlighting the potential of pathogen genome sequencing as a diagnostic tool. We document the genetic changes associated with adaptation to the hospital environment and with increasing drug resistance over time, and how MRSA evolution likely has been influenced by country-specific drug use regimens.
- Subjects :
- FIBRONECTIN-BINDING PROTEINS
High-level
Drug resistance
Antimicrobial resistance
medicine.disease_cause
VALENTINE LEUCOCIDIN GENE
NUCLEOTIDE-SEQUENCE
Pandemic
Cluster Analysis
Beta-lactamase
GENETICS & HEREDITY
Phylogeny
Genetics (clinical)
0303 health sciences
education.field_of_study
Great Britain
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
11 Medical And Health Sciences
Genomics
Staphylococcal Infections
3. Good health
Phylogeography
Valentine leucocidin gene
HIGH-LEVEL
Life Sciences & Biomedicine
Fibronectin-binding proteins
Methicillin-Resistant Staphylococcus aureus
Biochemistry & Molecular Biology
Genotype
Bioinformatics
Population
QH426 Genetics
FNB GENES
Biology
Staphylococcal infections
BETA-LACTAMASE
RS
Microbiology
Trimethoprim resistance
03 medical and health sciences
TRIMETHOPRIM RESISTANCE
Antibiotic resistance
Fusidic acid
Drug Resistance, Bacterial
Genetics
medicine
Humans
education
Pandemics
QH426
030304 developmental biology
Science & Technology
ANTIMICROBIAL RESISTANCE
030306 microbiology
Research
Outbreak
ANTIBIOTIC-RESISTANCE
06 Biological Sciences
medicine.disease
Methicillin-resistant Staphylococcus aureus
Virology
United Kingdom
FUSIDIC ACID
FNB genes
Nucleotide-sequence
Antibiotic-resistance
RS Pharmacy and materia medica
Genome, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 10889051
- Database :
- OpenAIRE
- Journal :
- Holden, M T G, Hsu, L-Y, Kurt, K, Weinert, L A, Mather, A E, Harris, S R, Strommenger, B, Layer, F, Witte, W, de Lencastre, H, Skov, R, Westh, H, Zemlickova, H, Coombs, G, Kearns, A M, Hill, R L R, Edgeworth, J, Gould, I, Gant, V, Cooke, J, Edwards, G F, McAdam, P, Templeton, K E, McCann, A, Zhou, Z, Castillo-Ramirez, S, Feil, E J, Hudson, L O, Enright, M C, Balloux, F, Aanensen, D M, Spratt, B G, Fitzgerald, J R, Parkhill, J, Achtman, M, Bentley, S D & Nübel, U 2013, ' A genomic portrait of the emergence, evolution, and global spread of a methicillin resistant Staphylococcus aureus pandemic ', Genome Research, vol. 23, no. 4, pp. 653-664 . https://doi.org/10.1101/gr.147710.112, Genome Research
- Accession number :
- edsair.doi.dedup.....0e478e9614bfb81a2f5ee25c07acf2e2