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Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2
- Source :
- The EMBO Journal
- Publication Year :
- 1998
-
Abstract
- We have identified an RNA-specific adenosine deaminase (termed Tad1p/scADAT1) from Saccharomyces cerevisiae that selectively converts adenosine at position 37 of eukaryotic tRNAAla to inosine. The activity of purified recombinant Tad1p depends on the conformation of its tRNA substrate and the enzyme was found to be inactive on all other types of RNA tested. Mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNAAla is not modified at position A37. Transformation of the mutant cells with the TAD1 gene restored enzyme activity. Tad1p has significant sequence similarity with the mammalian editing enzymes which act on specific precursor-mRNAs and on long double-stranded RNA. These findings suggest an evolutionary link between pre-mRNA editing and tRNA modification.
- Subjects :
- TRNA modification
Adenosine Deaminase
Molecular Sequence Data
Saccharomyces cerevisiae
Biology
General Biochemistry, Genetics and Molecular Biology
Substrate Specificity
Adenosine deaminase
RNA, Transfer
medicine
RNA Precursors
Amino Acid Sequence
RNA, Messenger
Molecular Biology
DNA Primers
chemistry.chemical_classification
General Immunology and Microbiology
Base Sequence
Sequence Homology, Amino Acid
General Neuroscience
Intron
RNA
Adenosine
Enzyme
chemistry
Biochemistry
RNA editing
Transfer RNA
biology.protein
RNA Editing
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....0e3e13df2667aeb81e1794d6ef8e5b4a