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Selective estrogen receptor modulators inhibit the effects of insulin-like growth factors in hyperparathyroidism
Selective estrogen receptor modulators inhibit the effects of insulin-like growth factors in hyperparathyroidism
- Source :
- Surgery. 132(6)
- Publication Year :
- 2002
-
Abstract
- Background. Primary hyperparathyroidism (HPT) predominately affects perimenopausal women, leading to speculations that an estrogen imbalance may be liable. We have previously demonstrated the importance of the insulin-like growth factor (IGF) axis in HPT. Because the antiestrogen tamoxifen has been shown to modulate the IGF axis, we examined the interactions of selective estrogen receptor modulators (SERMs) and IGF in HPT. Methods. Estrogen receptors were evaluated by Western immunoligand blotting. Sixteen parathyroid glands from 19 patients were included. After adhesion, the cells were treated with IGF (I or II) ± estrogen ± SERMs (tamoxifen, ICI 182,780) for 96 hours in serum-free media. Proliferation was assessed by measuring tritiated thymidine incorporation. Results. Both primary and secondary HPT express estrogen receptors α and β. Primary and secondary HPT had comparable responses to SERMs, they were analyzed together. Compared with control (100%), IGFs (I and II) induced a significant increase in DNA synthesis. Estradiol at 10−8 and 10−7 mol/L (physiologic range) had no significant effects on IGF (I and II, P > .05). Both tamoxifen and ICI 182,780 inhibited basal DNA synthesis (P < .05) and abolished the effects of both IGF I and II (P < .05). Conclusions. SERMs are capable of reducing basal and IGF-stimulated DNA synthesis. This reduction in proliferation has implications for cancer biology and therapeutic potential for SERMs in HPT.
- Subjects :
- Adenoma
Adult
Male
Selective Estrogen Receptor Modulators
medicine.medical_specialty
medicine.drug_class
medicine.medical_treatment
Blotting, Western
Estrogen receptor
Insulin-like growth factor
Radioligand Assay
Insulin-Like Growth Factor II
Internal medicine
medicine
Tumor Cells, Cultured
Estrogen Receptor beta
Humans
Insulin-Like Growth Factor I
Receptor
Aged
DNA synthesis
Estradiol
business.industry
Hyperparathyroidism
Estrogen Receptor alpha
Middle Aged
Antiestrogen
Tamoxifen
Endocrinology
Parathyroid Neoplasms
Receptors, Estrogen
Selective estrogen receptor modulator
Estrogen
Surgery
Female
business
hormones, hormone substitutes, and hormone antagonists
Cell Division
medicine.drug
Subjects
Details
- ISSN :
- 00396060
- Volume :
- 132
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Surgery
- Accession number :
- edsair.doi.dedup.....0e3c6fc40837ab38b2c4f3d914985f00