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Synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius
- Source :
- Enzyme and Microbial Technology. 26:348-358
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- Allysine ethylene acetal [(S)-2-amino-5-(1,3-dioxolan-2-yl)-pentanoic acid (2)] was prepared from the corresponding keto acid by reductive amination using phenylalanine dehydrogenase (PDH) from Thermoactinomyces intermedius ATCC 33205. Glutamate, alanine, and leucine dehydrogenases, and PDH from Sporosarcina species (listed in order of increasing effectiveness) also gave the desired amino acid but were less effective. The reaction requires ammonia and NADH. NAD produced during the reaction was recyled to NADH by the oxidation of formate to CO(2) using formate dehydrogenase (FDH). PDH was produced by growth of T. intermedius ATCC 33205 or by growth of recombinant Escherichia coli or Pichia pastoris expressing the Thermoactinomyces enzyme. Using heat-dried T. intermedius as a source of PDH and heat-dried Candida boidinii SC13822 as a source of FDH,98%, but production of T. intermedius could not be scaled up. Using heat-dried recombinant E. coli as a source of PDH and heat-dried Candida boidinii 98%. In a third generation process, heat-dried methanol-grown P. pastoris expressing endogenous FDH and recombinant Thermoactinomyces98% ee.
- Subjects :
- Alanine
biology
Bioengineering
Phenylacetaldehyde dehydrogenase
Formate dehydrogenase
biology.organism_classification
Applied Microbiology and Biotechnology
Biochemistry
Pichia pastoris
Thermoactinomyces
chemistry.chemical_compound
Phenylalanine dehydrogenase
chemistry
Formate
Leucine
Biotechnology
Subjects
Details
- ISSN :
- 01410229
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Enzyme and Microbial Technology
- Accession number :
- edsair.doi.dedup.....0e3a09021bd4305ecd5ef317f78de9a3