Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:19:59Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:44:50Z : No. of bitstreams: 1 2-s2.0-0034510558.pdf: 691173 bytes, checksum: 1736dcd6be20d2aefbe5a6897b8cb422 (MD5) Made available in DSpace on 2014-05-27T11:19:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-12-01 In this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci. Department of Biology Dalhousie University, Halifax, NS B3H 4J1 Departamento de Genktica e Evoluqlo Universidade Federal de Slo Carlos, Slo Carlos, SP Departamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SP Departamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SP