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Localization of copper and copper transporters in the human brain
- Source :
- Metallomics : integrated biometal science. 5(1)
- Publication Year :
- 2012
-
Abstract
- Disturbances in brain copper result in rare and severe neurological disorders and may play a role in the pathogenesis and progression of multiple neurodegenerative diseases. Our current understanding of mammalian brain copper transport is based on model systems outside the central nervous system and no data are available regarding copper transport systems in the human brain. To address this deficit, we quantified regional copper concentrations and examined the distribution and cellular localization of the copper transport proteins Copper transporter 1, Atox1, ATP7A, and ATP7B in multiple regions of the human brain using inductively coupled plasma-mass spectrometry, Western blot and immunohistochemistry. We identified significant relationships between copper transporter levels and brain copper concentrations, supporting a role for these proteins in copper transport in the human brain. Interestingly, the substantia nigra contained twice as much copper than that in other brain regions, suggesting an important role for copper in this brain region. Furthermore, ATP7A levels were significantly greater in the cerebellum, compared with other brain regions, supporting an important role for ATP7A in cerebellar neuronal health. This study provides novel data regarding copper regulation in the human brain, critical to understand the mechanisms by which brain copper levels can be altered, leading to neurological disease. © 2013 The Royal Society of Chemistry.
- Subjects :
- Central nervous system
ATP7A
Blotting, Western
Biophysics
chemistry.chemical_element
Biology
Biochemistry
Mass Spectrometry
Analytical Chemistry
Biomaterials
ATOX1
Copper-transporting ATPases
Copper Transport Proteins
medicine
Humans
Cation Transport Proteins
Cellular localization
Copper Transporter 1
Adenosine Triphosphatases
Brain Chemistry
Metals and Alloys
Human brain
medicine.disease
Copper
Immunohistochemistry
Cell biology
Metallochaperones
medicine.anatomical_structure
chemistry
Chemistry (miscellaneous)
Copper-Transporting ATPases
Menkes disease
Molecular Chaperones
Subjects
Details
- ISSN :
- 1756591X
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Metallomics : integrated biometal science
- Accession number :
- edsair.doi.dedup.....0e2b98b20172333778ca1c28bf8b8428