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Antimicrobial Peptide Mimicking Primary Amine and Guanidine Containing Methacrylamide Copolymers Prepared by Raft Polymerization
- Source :
- Biomacromolecules, vol 16, iss 12
- Publication Year :
- 2015
-
Abstract
- Naturally occurring antimicrobial peptides (AMPs) display the ability to eliminate a wide variety of bacteria, without toxicity to the host eukaryotic cells. Synthetic polymers containing moieties mimicking lysine and arginine components found in AMPs have been reported to show effectiveness against specific bacteria, with the mechanism of activity purported to depend on the nature of the amino acid mimic. In an attempt to incorporate the antimicrobial activity of both amino acids into a single water-soluble copolymer, a series of copolymers containing lysine mimicking aminopropyl methacrylamide (APMA) and arginine mimicking guanadinopropyl methacrylamide (GPMA) were prepared via aqueous RAFT polymerization. Copolymers were prepared with varying ratios of the comonomers, with degree of polymerization of 35-40 and narrow molecular weight distribution to simulate naturally occurring AMPs. Antimicrobial activity was determined against Gram-negative and Gram-positive bacteria under conditions with varying salt concentration. Toxicity to mammalian cells was assessed by hemolysis of red blood cells and MTT assays of MCF-7 cells. Antimicrobial activity was observed for APMA homopolymer and copolymers with low concentrations of GPMA against all bacteria tested, with low toxicity toward mammalian cells.
- Subjects :
- Erythrocytes
Polymers and Plastics
Cell Survival
Polymers
Antimicrobial peptides
Bioengineering
Peptide
Degree of polymerization
Arginine
Guanidines
Hemolysis
Article
Polymerization
Biomaterials
chemistry.chemical_compound
Engineering
Polymer chemistry
Materials Chemistry
Copolymer
Methacrylamide
Humans
Reversible addition−fragmentation chain-transfer polymerization
Amines
chemistry.chemical_classification
Acrylamides
Molecular Structure
Lysine
Biological Sciences
Amino acid
chemistry
Chemical Sciences
MCF-7 Cells
Peptidomimetics
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules, vol 16, iss 12
- Accession number :
- edsair.doi.dedup.....0df86630b21002538d7488179ebd6b3a