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Development of a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for nitroimidazoles in edible animal tissues and feeds
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 120:84-91
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The misuse of nitroimidazoles (NDZs) can lead to NDZs residues in edible animal tissues, which would be harmful to consumer health. To quickly monitor NDZs residues in edible animal tissues and feed, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with a simple sample preparation method and clean-up was developed in the present study. At first, a broad-specificity monoclonal antibody, 1D5, against NDZs has been produced, which the IC50 values of the NDZs, dimetridazole, ipronidazole, ronidazole hydroxydimetridazole, and hydroxyipronidazole, were 4.79μgL(-1), 0.47μgL(-1), 5.97μgL(-1), 23.48μgL(-1), and 15.03μgL(-1), respectively. The limit of detection of the method for the NDZ matrix calibration ranged from 4.2μgkg(-1) to 50.3μgkg(-1) in the feed matrices and from 0.11μgkg(-1) to 4.11μgkg(-1) in the edible animal tissues matrices. The recoveries of the NDZs were in the range of 75.5-111.8%. The CVs were less than 14.4%. A good correlation (r=0.9905) between the ELISA and HPLC-MS results of the tissues demonstrated the reliability of the developed ic-ELISA, which makes it a useful tool for screening of NDZs in animal edible tissue and feed.
- Subjects :
- Meat
Animal feed
medicine.drug_class
Clinical Biochemistry
Pharmaceutical Science
Enzyme-Linked Immunosorbent Assay
Food Contamination
02 engineering and technology
Monoclonal antibody
Sensitivity and Specificity
01 natural sciences
Analytical Chemistry
Mice
chemistry.chemical_compound
Antibody Specificity
Drug Discovery
medicine
Animals
Spectroscopy
Detection limit
Mice, Inbred BALB C
Chromatography
biology
010401 analytical chemistry
Antibodies, Monoclonal
021001 nanoscience & nanotechnology
Animal Feed
Dimetridazole
0104 chemical sciences
chemistry
Nitroimidazoles
Monoclonal
biology.protein
Female
Antibody
0210 nano-technology
Ipronidazole
Ronidazole
medicine.drug
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....0df07a3a6322d98c935fd18e5700c315