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E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration
- Source :
- Journal of Biomechanics. 71:159-166
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Collective cell movement is critical in pathological processes such as wound healing and cancer invasion. It entails complex interactions between adjacent cells and between cells-extracellular matrices. Most studies measure the migration patterns and force propagation by placing cells on flat, patterned substrates. The cooperative behavior resulting from cell-cell interactions is not well understood. We have developed a multi-channel microfluidic device that has junctional protein E-cadherin coated onto the sidewalls of the channels that enables the cells' lateral interactions with their neighbors to be studied. Our study reveals that epithelial cells rely on lateral E-cadherin-based adhesions to maintain the cohesion of the group. Cells move faster in narrower channels, but the average velocity along the channels is reduced in E-cadherin coated channels versus non-adhesive channels. We have directly measured the forces in the cross-linking protein, alpha-actinin, using FRET sensors during cell migration, and found that higher tension exists at the cell edges adjacent to the walls coated with E-cadherin, the implication being E-cadherin transmits the shear forces but does not provide a driving force for this migration.
- Subjects :
- 0301 basic medicine
Cell signaling
Shear force
Biomedical Engineering
Biophysics
Cell Communication
Actinin
Madin Darby Canine Kidney Cells
03 medical and health sciences
Dogs
0302 clinical medicine
Cell Movement
Lab-On-A-Chip Devices
Cell Adhesion
Shear stress
Animals
Orthopedics and Sports Medicine
Cell adhesion
Tension (physics)
Cadherin
Chemistry
Rehabilitation
Epithelial Cells
Cell migration
Cadherins
030104 developmental biology
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 00219290
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Biomechanics
- Accession number :
- edsair.doi.dedup.....0dd61154a8b9373ce5002be3fbbb9a00