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Photodynamic Approach for Teratoma-Free Pluripotent Stem Cell Therapy Using CDy1 and Visible Light
- Source :
- ACS Central Science, Vol 2, Iss 9, Pp 604-607 (2016), ACS Central Science
- Publication Year :
- 2016
- Publisher :
- American Chemical Society, 2016.
-
Abstract
- Pluripotent stem cells (PSC) are promising resources for regeneration therapy, but teratoma formation is one of the critical problems for safe clinical application. After differentiation, the precise detection and subsequent elimination of undifferentiated PSC is essential for teratoma-free stem cell therapy, but a practical procedure is yet to be developed. CDy1, a PSC specific fluorescent probe, was investigated for the generation of reactive oxygen species (ROS) and demonstrated to induce selective death of PSC upon visible light irradiation. Importantly, the CDy1 and/or light irradiation did not negatively affect differentiated endothelial cells. The photodynamic treatment of PSC with CDy1 and visible light irradiation confirmed the inhibition of teratoma formation in mice, and suggests a promising new approach to safe PSC-based cell therapy.<br />Stain, shine, and kill: CDy1 selectively stains pluripotent stem cells and, upon shining visible light, kills only stem cells without affecting differentiated cells, opening the possibility of teratoma-free stem cell therapy.
- Subjects :
- 0301 basic medicine
endocrine system diseases
General Chemical Engineering
medicine.medical_treatment
Biology
digestive system
Cell therapy
lcsh:Chemistry
03 medical and health sciences
medicine
Induced pluripotent stem cell
chemistry.chemical_classification
Reactive oxygen species
Regeneration (biology)
digestive, oral, and skin physiology
Visible light irradiation
General Chemistry
Stem-cell therapy
medicine.disease
digestive system diseases
030104 developmental biology
chemistry
lcsh:QD1-999
Cancer research
Teratoma
Research Article
Visible spectrum
Subjects
Details
- Language :
- English
- ISSN :
- 23747951 and 23747943
- Volume :
- 2
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- ACS Central Science
- Accession number :
- edsair.doi.dedup.....0dc5d0091230f0380623a9f07dcadd4f