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Homeostatic scaling is driven by a translation-dependent degradation axis that recruits miRISC remodeling
- Source :
- PLoS Biology, PLoS Biology, Vol 19, Iss 11, p e3001432 (2021)
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- Homeostatic scaling in neurons has been attributed to the individual contribution of either translation or degradation; however, there remains limited insight toward understanding how the interplay between the two processes effectuates synaptic homeostasis. Here, we report that a codependence between protein synthesis and degradation mechanisms drives synaptic homeostasis, whereas abrogation of either prevents it. Coordination between the two processes is achieved through the formation of a tripartite complex between translation regulators, the 26S proteasome, and the miRNA-induced silencing complex (miRISC) components such as Argonaute, MOV10, and Trim32 on actively translating transcripts or polysomes. The components of this ternary complex directly interact with each other in an RNA-dependent manner. Disruption of polysomes abolishes this ternary interaction, suggesting that translating RNAs facilitate the combinatorial action of the proteasome and the translational apparatus. We identify that synaptic downscaling involves miRISC remodeling, which entails the mTORC1-dependent translation of Trim32, an E3 ligase, and the subsequent degradation of its target, MOV10 via the phosphorylation of p70 S6 kinase. We find that the E3 ligase Trim32 specifically polyubiquitinates MOV10 for its degradation during synaptic downscaling. MOV10 degradation alone is sufficient to invoke downscaling by enhancing Arc translation through its 3′ UTR and causing the subsequent removal of postsynaptic AMPA receptors. Synaptic scaling was occluded when we depleted Trim32 and overexpressed MOV10 in neurons, suggesting that the Trim32-MOV10 axis is necessary for synaptic downscaling. We propose a mechanism that exploits a translation-driven protein degradation paradigm to invoke miRISC remodeling and induce homeostatic scaling during chronic network activity.<br />Homeostatic plasticity in neurons has been separately linked to translation or proteasomal degradation. This study reveals that RNA-dependent synergy between translation, degradation and miRISC remodeling is needed to achieve synaptic homeostasis during chronic changes in network activity.
- Subjects :
- Physiology
Gene Expression
Neural Homeostasis
Protein Synthesis
Biochemistry
Hippocampus
Rats, Sprague-Dawley
Tripartite Motif Proteins
RNA interference
Animal Cells
Protein biosynthesis
Medicine and Health Sciences
Homeostasis
Phosphorylation
Biology (General)
Polyubiquitin
Neurons
Neuronal Plasticity
Arc (protein)
Synaptic scaling
biology
Chemistry
General Neuroscience
EIF4E
Ribosomal Protein S6 Kinases, 70-kDa
Brain
Chemical Synthesis
Translation (biology)
Argonaute
Precipitation Techniques
Ubiquitin ligase
Cell biology
Electrophysiology
Nucleic acids
Genetic interference
Homeostatic Mechanisms
Epigenetics
Cellular Types
Cellular Structures and Organelles
Anatomy
General Agricultural and Biological Sciences
Research Article
Proteasome Endopeptidase Complex
Biosynthetic Techniques
QH301-705.5
Ubiquitin-Protein Ligases
Neurophysiology
Nerve Tissue Proteins
Mechanistic Target of Rapamycin Complex 1
Protein degradation
Research and Analysis Methods
General Biochemistry, Genetics and Molecular Biology
Developmental Neuroscience
Polysome
Genetics
Animals
RNA-Induced Silencing Complex
Immunoprecipitation
Receptors, AMPA
General Immunology and Microbiology
Ubiquitination
Biology and Life Sciences
Proteins
Protein Complexes
Proteasomes
Cell Biology
Primer
Cytoskeletal Proteins
MicroRNAs
Proteostasis
Proteasome
Cellular Neuroscience
Polyribosomes
Protein Biosynthesis
Proteolysis
Synapses
biology.protein
RNA
Protein Translation
Physiological Processes
Ribosomes
Transcription Factors
Synaptic Plasticity
Neuroscience
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PLoS Biology, PLoS Biology, Vol 19, Iss 11, p e3001432 (2021)
- Accession number :
- edsair.doi.dedup.....0da4a88c2fd24fdbcb7739baf43e87ef
- Full Text :
- https://doi.org/10.1101/2020.04.01.020164