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O-GlcNAcylation modulates PKA-CREB signaling in a manner specific to PKA catalytic subunit isoforms
- Source :
- Biochemical and Biophysical Research Communications. 497:194-199
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- O-GlcNAcylation is a post-translational modification of proteins. Protein kinase A (PKA)-cAMP response element binding protein (CREB) signaling plays critical roles in multiple biological processes. Isoforms α and β of PKA catalytic subunit (PKAc) and CREB are modified by O-GlcNAcylation. In the present study, we determined the role of O-GlcNAcylation in PKAc isoform-specific CREB signaling. We found that up-regulation of O-GlcNAcylation enhanced CREB phosphorylation, but suppressed CREB expression in exogenous PKAc isoform-unspecific manner. PKAc isoforms affected exogenous expression of OGT or OGA and protein O-GlcNAcylation differently. Up-regulation of O-GlcNAcylation did not significantly affect net PKAcα-CREB signaling, but enhanced PKAcβ-CREB signaling. The role of O-GlcNAcylation in PKA-CREB signaling was desensitized by insulin treatment. This study suggests a role of O-GlcNAcylation in PKA-CREB signaling by affecting phosphorylation of CREB in a PKAc isoform-specific manner.
- Subjects :
- 0301 basic medicine
Gene isoform
Acylation
medicine.medical_treatment
Protein subunit
Response element
Biophysics
N-Acetylglucosaminyltransferases
CREB
Biochemistry
Cell Line
Mice
03 medical and health sciences
Catalytic Domain
medicine
Animals
Humans
Phosphorylation
Cyclic AMP Response Element-Binding Protein
Protein kinase A
Molecular Biology
Neurons
biology
Chemistry
Insulin
Binding protein
Cell Biology
Cyclic AMP-Dependent Protein Kinases
Cell biology
Isoenzymes
HEK293 Cells
030104 developmental biology
biology.protein
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 497
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....0d9d2f402326e35d8576d68ad0b0af9f