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Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiri
- Source :
- Modern Pathology
- Publication Year :
- 2013
- Publisher :
- Nature Publishing Group, 2013.
-
Abstract
- Idiopathic pulmonary fibrosis is a fatal disease without effective therapy or diagnostic test. To investigate a\ud potential role for c�herpesviruses in this disease, 21 paraffin-embedded lung biopsies from patients diagnosed\ud with idiopathic pulmonary fibrosis and 21 lung biopsies from age-matched controls with pulmonary fibrosis of\ud known etiology were examined for a series of c�herpesviruses’ DNA/RNA and related proteins using in situ\ud hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR)-based methods. We detected four\ud proteins known to be in the genome of several c�herpesviruses (cyclin D, thymidylate synthase, dihydrofolate\ud reductase, and interleukin-17) that were strongly co-expressed in the regenerating epithelial cells of each of the\ud 21 idiopathic pulmonary fibrosis cases and not in the benign epithelia of the controls. Among the c�\ud herpesviruses, only herpesvirus saimiri expresses all four of these ‘pirated’ mammalian proteins. We found\ud herpesvirus saimiri DNA in the regenerating epithelial cells of 21/21 idiopathic pulmonary fibrosis cases using\ud four separate probe sets but not in the 21 controls. RT-PCR showed that the source of the cyclin D RNA in active\ud idiopathic pulmonary fibrosis was herpesvirus saimiri and not human. We cloned and sequenced part of\ud genome corresponding to the DNA polymerase herpesvirus saimiri gene from an idiopathic pulmonary fibrosis\ud sample and it matched 100% with the published viral sequence. These data are consistent with idiopathic\ud pulmonary fibrosis representing herpesvirus saimiri-induced pulmonary fibrosis. Thus, treatment directed\ud against viral proliferation and/or viral-associated proteins may halt disease progression. Further, demonstration\ud of the viral nucleic acids or proteins may help diagnose the disease.
- Subjects :
- Male
Pathology
medicine.medical_specialty
DNA polymerase
Cyclin D
viruses
In situ hybridization
Q1
Pathology and Forensic Medicine
Herpesvirus 2, Saimiriine
herpesvirus saimiri
Idiopathic pulmonary fibrosis
Pulmonary fibrosis
medicine
Humans
cyclin D
Gene
In Situ Hybridization
Aged
Lung
biology
Reverse Transcriptase Polymerase Chain Reaction
RNA
Herpesviridae Infections
Middle Aged
medicine.disease
idiopathic pulmonary fibrosis
R1
IL-17
medicine.anatomical_structure
Immunology
DNA, Viral
biology.protein
Original Article
Female
Subjects
Details
- Language :
- English
- ISSN :
- 15300285 and 08933952
- Volume :
- 27
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Modern Pathology
- Accession number :
- edsair.doi.dedup.....0d9ca3aa43587defc9b4efb2693719c2