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Optogenetic Stimulation of GABA Neurons can Decrease Local Neuronal Activity While Increasing Cortical Blood Flow
- Source :
- Journal of Cerebral Blood Flow & Metabolism. 35:1579-1586
- Publication Year :
- 2015
- Publisher :
- SAGE Publications, 2015.
-
Abstract
- We investigated the link between direct activation of inhibitory neurons, local neuronal activity, and hemodynamics. Direct optogenetic cortical stimulation in the sensorimotor cortex of transgenic mice expressing Channelrhodopsin-2 in GABAergic neurons (VGAT-ChR2) greatly attenuated spontaneous cortical spikes, but was sufficient to increase blood flow as measured with laser speckle contrast imaging. To determine whether the observed optogenetically evoked gamma aminobutyric acid (GABA)-neuron hemodynamic responses were dependent on ionotropic glutamatergic or GABAergic synaptic mechanisms, we paired optogenetic stimulation with application of antagonists to the cortex. Incubation of glutamatergic antagonists directly on the cortex (NBQX and MK-801) blocked cortical sensory evoked responses (as measured with electroencephalography and intrinsic optical signal imaging), but did not significantly attenuate optogenetically evoked hemodynamic responses. Significant light-evoked hemodynamic responses were still present after the addition of picrotoxin (GABA-A receptor antagonist) in the presence of the glutamatergic synaptic blockade. This activation of cortical inhibitory interneurons can mediate large changes in blood flow in a manner that is by and large not dependent on ionotropic glutamatergic or GABAergic synaptic transmission. This supports the hypothesis that activation of inhibitory neurons can increase local cerebral blood flow in a manner that is not entirely dependent on levels of net ongoing neuronal activity.
- Subjects :
- Male
Neurotransmission
Biology
Inhibitory postsynaptic potential
gamma-Aminobutyric acid
GABA Antagonists
Mice
Glutamatergic
Channelrhodopsins
Interneurons
Quinoxalines
medicine
Animals
Premovement neuronal activity
GABAergic Neurons
gamma-Aminobutyric Acid
Lasers
Electroencephalography
GABA receptor antagonist
Optogenetics
nervous system
Neurology
Cerebrovascular Circulation
GABAergic
Original Article
Female
Neurology (clinical)
Dizocilpine Maleate
Cardiology and Cardiovascular Medicine
Excitatory Amino Acid Antagonists
Neuroscience
Photic Stimulation
medicine.drug
Ionotropic effect
Subjects
Details
- ISSN :
- 15597016 and 0271678X
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Cerebral Blood Flow & Metabolism
- Accession number :
- edsair.doi.dedup.....0d9aafdf280d5a0f0fb485d7bba07c3e